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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (8): 63-67.

所属专题： 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

响应面法优化胶冻样芽孢杆菌发酵条件的研究

王鸿磊 王红艳 刘鹏丽 崔丛光   

  • 收稿日期:2011-12-09 修回日期:2012-01-06 出版日期:2012-03-15 发布日期:2012-03-15
  • 基金资助:

    烟台市科技攻关

Optimization of the Fermentation Conditions of Bacillus mucilaginosus Using Response Surface Method

  • Received:2011-12-09 Revised:2012-01-06 Online:2012-03-15 Published:2012-03-15

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摘要：

为了优化响应面法对胶冻样芽孢杆菌的发酵条件。在单因素的基础上选择酵母膏、糖蜜以及pH为自变量，芽孢杆菌的芽孢数为响应值，根据Box-Behnken实验设计原理进行响应面法分析，确定了胶冻样芽孢杆菌发酵条件的最佳组成为：酵母膏0.53%，糖蜜2.18%，pH 7.91，在此条件下最大理论芽孢数为1.853×108个/mL。经过3次平行实验验证，实际芽孢数为1.813×108个/mL。该试验值与预测值很接近。

关键词: 身体质量指数/精液品质/生殖激素, 身体质量指数/精液品质/生殖激素

Abstract:

Response surface method was used to optimize the fermentation conditions of Bacillus mucilaginosus. Experiment factors and levels were firstly selected by one-factor tests. On the basic of the previous results and according to the Box-Behnken experimental design principles, the method of response surface method with 3 factors and 3 levels was adopted. Then the factors influencing were determined by means of regression analysis. After analysis on each significant and interaction effect, the results showed that the optimum fermentation conditions of Bacillus mucilaginosus as follows: yeast extract 0.53%, molasses 2.18%, pH 7.91. Then the largest number of bacteria predicted by the theoretical model was 1.853×108 cfu. After fermentation conditions optimization, the error of test value and predictive value was 0.7%. The experimental data and the predicted value of model fit well.