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中国农学通报 ›› 2012, Vol. 28 ›› Issue (4): 263-268.

• 林学 园艺 园林 • 上一篇    下一篇

次氯酸钠用于植物培养基灭菌方法初探

张雨婷 王贤 王永勤   

  • 收稿日期:2011-12-15 修回日期:2012-01-23 出版日期:2012-02-05 发布日期:2012-02-05
  • 基金资助:

    北京市农林科学院科技创新能力建设专项(KJCX201101010)

Sodium hypochlorite for sterilization Methods of plant culture medium

  • Received:2011-12-15 Revised:2012-01-23 Online:2012-02-05 Published:2012-02-05

摘要:

对在培养基的配制过程中添加次氯酸钠替代培养基高压蒸汽灭菌的方法进行了研究,取得了如下结果:在培养基凝固后添加次氯酸钠,温度25℃、光照强度2000lx、光照时间为16小时的培养条件下,浓度为360mg/L时,仍有部分培养基污染;在培养基配制过程中,琼脂加热溶解后,趁热在培养基中添加10mg/L次氯酸钠,未发污染。以‘神马’菊花为外植体,在培养基配制过程中加入终浓度20mg/L的次氯酸钠时,植株可以正常生长,而且,生长状况好于高压灭菌组;次氯酸钠浓度高于50 mg/L时,生长状况差于高压灭菌组,而且会产生伤害。

关键词: 半农半牧区, 半农半牧区

Abstract:

he preparation process in the medium instead of sodium hypochlorite added to the medium pressure steam sterilization methods were studied. The result were as following : hypochlorite in the medium added after solidification, 25 ℃, 2000lx light intensity, 16 hours illumination of culture conditions, the concentration of 360mg / L, some parts of the culture medium were still polluted. During the media preparation process, the dissolved agar heated, hot in the medium supplemented with 10mg / L sodium hypochlorite, no polluted culture medium was found. With 'God Ma' Chrysanthemum explants in culture medium during the preparation of the final concentration 20mg / L of sodium hypochlorite,all the plants grew normally, furthermore, their growth conditions were better than that of the group of the high-pressure sterilization. When sodium hypochlorite concentrations was above 50 mg / L, the growth was not only more poor than the control, but also did harm to them. The results show that the proper concentration of sodium hypochlorite instead of high-pressure steam sterilization is effective.

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