血管内皮生长因子及其受体在胚胎卵黄囊
不同发育时期的表达

中国农学通报 ›› 2005, Vol. 21 ›› Issue (6): 8-8.

血管内皮生长因子及其受体在胚胎卵黄囊不同发育时期的表达

王跃嗣,,姜昱竹,,李建远,王海燕,范光丽

出版日期:2005-06-05 发布日期:2005-06-05

Research on the Expression of Vascular Endothelial Growth Factor and its Receptor in Human Yolk Sac at Various Developmental Stages

Wang Yuesi, Jiang Yuzhu, Li Jianyuan, Wang Haiyan, Fan Guangli

Online:2005-06-05 Published:2005-06-05

摘要/Abstract

摘要： 为研究胚胎不同时期VEGF、KDR和CD34在人卵黄囊中的表达情况，了解胚胎造血的发育过程和机理。采用免疫组织化学SP法卵黄囊冰冻切片进行染色，光镜观察。结果发现在第3~4周的人卵黄囊低表达VEGF和KDR，不表达CD34。4~6周组强表达VEGF、KDR和CD34。在血岛内阳性细胞大而圆，成簇或分散聚集，有些细胞沿血岛边缘形成血管样结构。6周以后，卵黄囊弱表达VEGF、KDR和CD34。上述结果提示卵黄囊可表达造血和血管生成相关因子，随胚胎发育呈阶段性表达。卵黄囊中出现造血干细胞和血管内皮细胞的分化。

Abstract: To investigate the expression of VEGF, KDR and CD34 in human yolk sac at different stages, we applied immunohistochemical staining and light microscope. It was demonstrated low expression of VEGF, KDR and null expression of CD34 at 3~4 week human yolk sac, strong expression of VEGF, KDR and CD34 at 4~6 week group. Positive cells in the blood island presented large and round, cluster-like or dispersed. And some cells surrounding the blood island showed blood vessel-like structure. At more than 6 weeks after gestation group, the yolk sac expressed VEGF, KDR and CD34 weakly. These findings suggested that the yolk sac could express hematopoietic and angiogenetic associated factors with embryonic development, and contained possibly the hemangioblast which could differentiate into hematopoietic stem cells and vascular endothelial cells.

王跃嗣,,姜昱竹,,李建远,王海燕,范光丽. 血管内皮生长因子及其受体在胚胎卵黄囊不同发育时期的表达[J]. 中国农学通报, 2005, 21(6): 8-8.

Wang Yuesi, Jiang Yuzhu, Li Jianyuan, Wang Haiyan, Fan Guangli. Research on the Expression of Vascular Endothelial Growth Factor and its Receptor in Human Yolk Sac at Various Developmental Stages[J]. Chinese Agricultural Science Bulletin, 2005, 21(6): 8-8.

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