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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (25): 224-228.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

不同产地人参中4种脱氢酶活力比较

杨菲 赵雨 王思明 刘美辰 李晓华   

  • 收稿日期:2012-02-09 修回日期:2012-04-23 出版日期:2012-09-05 发布日期:2012-09-05

Comparison of Activity of four Dehydrogenases in Ginseng from Different Origins

  • Received:2012-02-09 Revised:2012-04-23 Online:2012-09-05 Published:2012-09-05

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摘要：

为了给人参的培育和优选提供理论依据，采用中性缓冲液提取粗酶液，应用分光光度法对15个不同产地的人参中苹果酸脱氢酶(Malate Dehydrogenase MDH)，乳酸脱氢酶(Lactate Dehydrogenase, LDH)，乙醇脱氢酶(Alcohol Dehydrogenase, ADH)，葡萄糖-6-磷酸脱氢酶(Glucose-6-phosphate Dehydrogenase, G6PDH）4种脱氢酶活力进行比较。结果表明不同产地人参脱氢酶活力差别明显，同一产地4种脱氢酶活力趋势基本相同。其中安图县万宝镇的人参样品的MDH、LDH、G6PDH 3种酶活力均是最高值，分别为124.58U/(g?Fw)、129.88 U/(g?Fw)、109.84U/(g?Fw)；黑龙江2个产地的4种酶活力普遍比较低。运用SPSS13.0软件对15批样品进行系统聚类分析，将样品分为4类。MDH、LDH、ADH、G6PDH的活力可以作为人参培育和优选提供理论依据。

关键词: 节肢动物群落, 节肢动物群落

Abstract:

The aim was to provide theoretical basis for the cultivation and optimization of ginseng. Adopt neutral buffer solution to extract the enzyme solution of Radix Ginseng. The activities of Malate Dehydrogenase (MDH), Lactate Dehydrogenase (LDH), Alcohol Dehydrogenase (ADH) and Glucose-6-phosphate Dehydrogenase (G6PDH) were detected by spectrophotometry, and compared. The clustering analysis was performed using the software SPSS13.0 to system for 15 batch sample. There were obvious differences of the activities dehydrogenase of ginseng from different origin. The activities of four dehydrogenases from the same origin were basically same. In Antu County Wanbao Town, MDH, LDH and G6PDH had the highest activities, 124.58 U/(g?Fw), 129.88 U/(g?Fw) and 109.84 U/(g?Fw) respectively. The four kinds of enzymes activity of two origins in Heilongjiang Province were generally low. The sample was divided into four categories. The activities of MDH, LDH, ADH and G6PDH could provide theoretical basis for the cultivation and optimization of ginseng.