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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (24): 288-292.

所属专题： 生物技术

• 食品 营养 检测 安全 • 上一篇    下一篇

Klyveromyces lactis酵母生产乳糖酶发酵条件的研究

钱卫东 付云芳 胡娜   

  • 收稿日期:2012-02-25 修回日期:2012-06-08 出版日期:2012-08-25 发布日期:2012-08-25
  • 基金资助:

    离子束介导黄芩苷DNA基因组转化酵母研究

Study on the Fermentation Conditions of the Yeast Kluyveromyces lactis Producing Lactase

  • Received:2012-02-25 Revised:2012-06-08 Online:2012-08-25 Published:2012-08-25

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摘要：

为了获得一株产乳糖酶乳酸克鲁维酵母(Kluyveromyces lactis ATCC 12426)的最佳发酵参数，通过正交试验优化了产乳糖酶的培养条件。结果表明：最佳种子培养基组分为：葡萄糖2%、酵母膏0.35%、蛋白胨0.35%。发酵培养基的最佳组分为：乳糖4%、葡萄糖2%、酵母膏0.18%、尿素0.15%、KH2PO4 1%、MgCl2 0.1%、MnCl2 0.01%。最佳发酵条件为：接种量5%，温度30℃，初始pH 7.0，摇床转速140 r/min，培养时间18 h。最佳条件下摇瓶发酵其胞内酶活高达20.861 U/mL。

关键词: XopN, XopN

Abstract:

To achieve the optimal fermentation conditions of the lactase-producing yeast Kluyveromyces lactis, the optimal cultural conditions were obtained using orthogonal design. The optimal seed medium compositions were as follows: glucose 2%, yeast extract 0.35%, peptone 0.35%. Meanwhile, the optimal compositions of fermentation medium were as follows: lactose 4%, glucose 2%, urea 0.15%, KH2PO4 1%, MgCl2 0.1%, MnCl2 0.01%. The cultural conditions were: inoculation size of 5%, at a temperature of 30℃, initial pH 7.0, shake flask rotation of 140 r/min and culture time of 18 h. Under these conditions, the lactase activity of K. lactis could reach 20.861 U/mL.