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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (22): 231-235.doi: 10.11924/j.issn.1000-6850.2012-0770

• 林学 园艺 园林 • 上一篇    下一篇

牡丹花芽休眠解除进程中碳氮比的变化与低温处理时间的关系

张晓玲 王春华 穆平 盖树鹏 郑国生   

  • 收稿日期:2012-03-07 修回日期:2012-04-20 出版日期:2012-08-05 发布日期:2012-08-05
  • 基金资助:

    外源激素辅助低温解除牡丹休眠技术研究与应用

Correlation Analysis Between C/N and the Amount of Chilling Treatment During the Release of Dormant Floral Buds in Paeonia suffruticosa

  • Received:2012-03-07 Revised:2012-04-20 Online:2012-08-05 Published:2012-08-05

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摘要：

以牡丹‘鲁荷红’花芽为材料，对不同低温处理的牡丹花芽的4种营养物质的含量进行测定，同时测定淀粉水解酶和谷氨酰胺合成酶的活性。目的是探索其变化趋势与牡丹休眠解除的相关性。试验结果表明，在牡丹花芽休眠解除过程中，淀粉、可溶性糖与可溶性蛋白质均有不同程度的增加；但是基本打破休眠时，即低温15天时可溶性蛋白含量有所降低，打破休眠后，又恢复含量；而游离氨基酸的量变化不明显，整个过程趋于稳定。其花芽的总体碳氮比，在未感受低温时，比值较低，感受低温后的花芽其总体的碳氮比持续处于较高的水平，并趋于稳定。在牡丹花芽休眠解除过程中，淀粉水解酶活性持续缓慢升高，可溶性糖含量升高，碳水化合物积累，是碳氮比升高的重要原因。谷氨酰胺合成酶活性呈现先升高后下降的趋势, 在感受低温15天时达到最高值。

关键词: 免疫细胞化学, 免疫细胞化学

Abstract:

Paeonia suffroticosa’ Lu He Hong’ was selected as plant materials in this research. During the dormancy release, the dynamic changes in contents of the endogenous soluble protein, soluble sugar, soluble starch and free amino acid and the activity of amylase and Glutamine synthase were measured in order to explore the physiological mechanism of dormancy releasing in tree peony, relevance to nutrients content. The results showed that contents of the soluble protein, soluble sugar, and soluble starch had varying degrees of increasing. After 15 days of chilling treatment, dormancy was broken and the content of the soluble protein decreased, then increased again. But the content of free amino acid had no significant difference from the control. Carbon and nitrogen ratio was lower with no chilling treatment. The ratio after chilling treatment was continuously increased and maintained at high level. Furthermore, the activity of amylase increased but the change trend was slow, with the content of soluble sugar increasing, to increase the accumulation of carbohydrates. The activity of Glutamine synthase showed the trend o f increasing firstly and lowering then, reaching the highest value under 15 days chilling treatment.