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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (23): 85-89.doi: 10.11924/j.issn.1000-6850.2012-1193

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

单增李斯特菌定量检测方法研究

杨松 曲祖乙 刘永华   

  • 收稿日期:2012-03-30 修回日期:2012-05-24 出版日期:2012-08-15 发布日期:2012-08-15
  • 基金资助:

    辽宁省教育厅科技研究基金

Detection of Listeria monocytogenes by Real-time Fluorescent PCR Assay

  • Received:2012-03-30 Revised:2012-05-24 Online:2012-08-15 Published:2012-08-15

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摘要：

为建立快速、准确、定量检测单增李斯特菌的方法，根据GenBank中单增李斯特菌hlyA基因序列设计引物，建立单增李斯特菌real-time PCR检测方法。标准曲线方程为Y=-3.311X +41.162，相关系数R2=0.991,扩增效率E =96.528%。Real-time PCR 检测单增李斯特菌具有快速、灵敏、高效的优点，适宜于食品中单增李斯特菌污染的调查及监测。

关键词: 水源涵养, 水源涵养

Abstract:

To develop a rapid and accurate real-time PCR method for detection of Listeria monocytogenes，hlyA gene of Listeria monocytogenes was used as target sequence to design primers.A new real-time PCR assay was developed. The standard curve was Y = -3．311X + 38.162，R2 =0.991． A real-time PCR assay for the detection of Listeria monocytogenes was developed，which supplied a technology platform for the rapid detection of Listeria monocytogenes．