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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (23): 1-5.doi: 10.11924/j.issn.1000-6850.2012-1216

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 •    下一篇

庄河大骨鸡ghr、igf-1基因的PCR-RFLP分析及基因聚合与产肉性能的关联分析研究

王洪才 袁晓春 宋天玉   

  • 收稿日期:2012-03-31 修回日期:2012-06-26 出版日期:2012-08-15 发布日期:2012-08-15
  • 基金资助:

    辽宁省教育厅科技项目“酸枣仁皂甙缓解肉仔鸡热应激作用机制的研究”

PCR-RFLP Analysis on ghr Gene, igf-1 Gene and Combination Effects on Meat Performance in Zhuanghe Dagu Chicken

  • Received:2012-03-31 Revised:2012-06-26 Online:2012-08-15 Published:2012-08-15

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摘要：

为了探究庄河大骨鸡ghr、igf-1基因聚合与产肉性能相关联的分子标记。利用PCR-RFLP标记技术和最小二乘法分析了ghr、igf-1的多态性及其2基因的聚合基因型与17周龄庄河大骨鸡产肉性能的关联。结果表明：ghr基因和igf-1基因均存在3种基因型且处在哈代温伯格平衡状态；聚合基因型GGTT、GHTT和GGKT的活重、屠体重、半净膛重、全净膛重、胸肌重、平均日增重显著的高于其他各基因型(P<0.05)。聚合基因型GGTT、GGKT、GHTT对庄河大骨鸡的产肉性能有显著影响，但是否能作为庄河大骨鸡选育的重要理论依据还有待进一步研究。

关键词: 生长点, 生长点

Abstract:

This study was designed to determine the relation between the meat performance of Zhuanghe Dagu Chicken and combination effects of ghr gene and igf-1 gene. PCR-RFLP technique was used to analysis the polymorphisms of ghr gene and igf-1 gene, while least square method was used to analysis the correlation between the meat performance of the 17 weeks old Zhuanghe Dagu chicken and specific genotypes as well as the combination effect of ghr, igf-1. It turned out that both of the genes have 3 different genotypes, the frequency of each genotype met the Hardy-weinberg equilibrium. The chickens with the combination genotype GGTT, GHTT and GGKT had significant better performances in terms of live weight, carcass weight, semi-eviscerated weight, eviscerated weight, pectorales weight, average daily gain than those with other genotypes (P<0.05). Above all, combination genotype GGTT, GGKT, GHTT of both ghr gene and igf-1 gene contribute significantly to the meat performance of the chickens. Further study was still needed to determine whether these specific genotypes could be taken advantage as candidates in the breeding application.

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