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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (28): 28-33.

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

几丁质酶及β-1,3-葡聚糖基因转化橡胶树的研究

黄天带 孙爱花 杨加伟 华玉伟 黄华孙   

  • 收稿日期:2012-04-23 修回日期:2012-06-07 出版日期:2012-10-05 发布日期:2012-10-05
  • 基金资助:

    国家自然科学基金;中国热带农业科学院橡胶研究所基本科研业务费专项资金;现代农业产业技术体系建设专项

Genetic Transformation of Chitinase and β-1, 3-Glucase Gene to Rubber Tree

  • Received:2012-04-23 Revised:2012-06-07 Online:2012-10-05 Published:2012-10-05

PDF (PC)

901

摘要：

旨在通过基因工程的方法提高橡胶树白粉病抗性。以高产、综合性状优良的大规模推广级品种‘热研7-33-97’15090块花药愈伤组织为受体，通过农杆菌介导将菜豆几丁质酶和烟草β-1,3-葡聚糖酶基因转入橡胶树，获得1386个抗性胚状体，其中277个通过次生体胚发生成功增殖。PCR结果表明有49个胚状体为阳性，转化效率为0.33%。本研究通过农杆菌介导将菜豆几丁质酶和烟草β-1,3-葡聚糖酶基因转入橡胶树，PCR阳性率为0.33%，为通过基因工程手段提高橡胶树白粉病抗性奠定了良好的基础。

关键词: 最优方案, 最优方案

Abstract:

The aim was to enhance the resistance of powdery mildew of rubber tree with genetic engineering. 15090 anther callus of high yield, large-scale spread, elite variety ‘CATAS 7-33-97’ was used as receptor in this study, bean chitinase and tobacco β-1,3-glucanase gene were transformed into Hevea mediated by Agrobacterium, 1386 resistant somatic embryos were differentiated, then proliferated by secondary embryogenesis, 277 were successful. PCR results indicated that 49 embryos were positive, with 0.33% transformation efficiency. Bean chitinase and tobacco β-1, 3-glucanase gene were transformed into Hevea mediated by Agrobacterium, with 0.33% PCR positive efficiency. It laid a good foundation for improving the powdery mildew resistance by genetic engineering in Hevea.