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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (31): 7-14.

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

巴西橡胶树膜结合NAC转录因子HbNTL1的克隆及生物信息学分析

康桂娟 曾日中 聂智毅 代龙军 段翠芳 黎瑜   

  • 收稿日期:2012-05-02 修回日期:2012-06-29 出版日期:2012-11-05 发布日期:2012-11-05
  • 基金资助:

    中国热带农业科学院橡胶研究所基本科研业务费资助

Cloning and Bioinformatics Analysis of a Membrane-bound NAC Transcription Factor Gene HbNTL1 from Hevea brasiliensis

  • Received:2012-05-02 Revised:2012-06-29 Online:2012-11-05 Published:2012-11-05

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摘要：

本实验根据巴西橡胶树胶乳cDNA文库中筛选到的NAC EST序列，克隆了橡胶树膜结合NAC转录因子，HbNTL1的cDNA和基因组DNA序列。生物信息学分析显示HbNTL1基因包含6个外显子和5个内含子，开放阅读框(ORF)为1704bp，编码了567个氨基酸。HbNTL1编码蛋白的相对分子量为62.52 kDa，理论等电点PI为4.62，N-端具有保守的NAC结构域，高度变异的C-端有一个跨膜结构域(TM)。序列比对和系统进化分析表明HbNTL1蛋白属于NAC转录因子家族中的NAC2亚族，推测其可能与橡胶树生长发育和胁迫应答有关。

关键词: Ni-Co镀层, Ni-Co镀层

Abstract:

The NAC (NAM, ATAF1, -2, and CUC2) gene family encodes a large family of plant-specific transcription factors that play diverse roles in plant development and stress regulation. Membrane-bound NAC transcription factors are a group of NAC transcription factors with transmembrane domain. In this study, the sequence of a membrane-bound NAC transcription factor, HbNTL1 was cloned from cDNA and genomic DNA based on the EST sequence of NAC isolated from the cDNA libraries of Hevea brasiliensis latex. Bioinformatics analysis shows that HbNTL1 contained 6 exons and 5 introns within the genomic DNA sequence and have an open reading frame of 1704 bp encoding a protein with 567 amino acids. The estimated molecular mass isoelectric point of HbNTL1 was 62.52 kDa and 4.62 respectively. There was a conserved NAC domain in the N-terminus and transmembrane domain (TM) in the highly divergent C-terminus. Sequence alignment and phylogenetic analysis revealed that HbNTL1 was a member of NAC transcription factor family belonging to NAC2 subgroup and probably play key roles in the development and stress responses in rubber tree.