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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (28): 239-244.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

栽培型与野生型青蒿愈伤组织及毛状根的诱导

赵欣 何利明 尹静 詹亚光 陈学武 武田 古丽   

  • 收稿日期:2012-05-09 修回日期:2012-07-09 出版日期:2012-10-05 发布日期:2012-10-05

Callus and hairy root induction from Artemisia annua of Cultivated and wild type

  • Received:2012-05-09 Revised:2012-07-09 Online:2012-10-05 Published:2012-10-05

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摘要：

为了比较栽培型与野生型引种青蒿愈伤组织及毛状根的诱导差异，以2种类型青蒿种子为材料，在不加激素的MS培养基中诱导青蒿苗，并利用青蒿的根、茎和叶片在MS+6-BA 0.5 mg/L+IBA 0.5 mg/L诱导愈伤组织，发根农杆菌C58C1、ATCC15834和Accc10600诱导毛状根。结果表明青蒿愈伤组织诱导效率以根最佳，其次为茎和叶片。农杆菌C58C1和ATCC15834分别以青蒿的根和叶片的诱导率可达100%和70%；Accc10600菌种不能诱导青蒿叶片毛状根发生，但是可诱导根生出毛根的芽点。该研究为青蒿素的生物反应器规模化生产奠定了基础。

关键词: 胚珠败育, 胚珠败育

Abstract:

In order to compare difference of hairy root and callus induction of the cultivated and wild type of Artemisia annua, with two types of Artemisia seeds as materials, induction southernwood seedlings in the MS medium without hormone, and root, stem and leaf of Artemisia annua were use induction callus in MS+6-BA 0.5 mg/L+IBA 0.5 mg/L, and Pseudomonas aeruginosa C58C1, ATCC15834 and Accc10600 were used induction hair root. The results showed that the callus induction efficiency best by root of Artemisia annua, followed by the stem and leaf. Aeruginosa C58C1 and ATCC15834 respectively in Artemisia annua root and leaf induction rate of hair roots was 100% and 70%; Accc10600 strain could not be induced hairy roots in Artemisia leaves, but can induce root appeared the hair roots buds point. The study laid the foundation for production of artemisinin by bioreactor.