欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (31): 243-246.

• 林学 园艺 园林 • 上一篇    下一篇

降低魔芋球茎组培褐变的技术研究

李川 员燕 杨松杰   

  • 收稿日期:2012-06-01 修回日期:2012-07-20 出版日期:2012-11-05 发布日期:2012-11-05

Study on Technical of Reducing Tissue Culture Browning of Amorphophallus Konjac Bulbs

  • Received:2012-06-01 Revised:2012-07-20 Online:2012-11-05 Published:2012-11-05

PDF (PC)

636

摘要：

以魔芋球茎为外植体，研究组织培养过程中有效降低外植体褐变的方法。采用MS+6-BA 1 mg/L+NAA 1 mg/L为基本培养基，用正交实验方法研究不同琼脂浓度、吸附剂及光、暗2种不同培养条件下，对魔芋组织培养过程中褐变的影响。在MS+6-BA 1 mg/L+NAA 1 mg/L+蔗糖30 g/L+琼脂 6 g/L+AC 0.5 g/L+PVP 0.05 g/L及暗培养条件下，魔芋外植体的褐变率减低为38.3%，愈伤组织诱导率提高到78.3%。在培养基中加入一定量的活性炭(AC)和聚乙烯吡咯烷酮(PVP)，并进行暗培养，可在一定程度上控制魔芋外植体的褐变，促进愈伤组织形成。

关键词: 意愿调查, 意愿调查

Abstract:

Using Amorphophallus konjac bulbs as explants, the research studied method of reducing browning of explants in tissue culture process. By using MS+6-BA 1 mg/L+ NAA 1 mg/L as the basic medium and the orthogonal experimental method to study the impact on browning with different agar concentrations, adsorbent and light, dark two kinds of different culture conditions. The results showed that the callus growth speed was enhanced and the browning of explants was under control in the combination of MS+ 6-BA 1 mg/L+ NAA 1 mg/L+ sugar 30 g/L+ agar 6 g/L+AC 0.5 g/L+PVP 0.05 g/L. It can make to reduce the browning rate of 38.3%, callus induction rate increased to 78.3%. The konjac bulbs explants the browning can be effectively controlled, which add a certain amount of activated carbon (AC) and polyvinylpyrrolidone (PVP) in the culture medium and in the dark conditions, its callus can be promptly formed.