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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (35): 59-64.

所属专题： 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

溶栓酶体内溶栓作用初探及其胶囊剂的制备

袁军 谢声勇 沈榕强 庄振宏 杨燕凌 林玲娜 林玲   

  • 收稿日期:2012-07-19 修回日期:2012-10-25 出版日期:2012-12-15 发布日期:2012-12-15
  • 基金资助:

    福建省科学技术厅自然科学基金项目;福建省教育厅科技项目

Primary Studies on Fibrinolytic Function of Fibrinolysis Enzyme in vivo and Preparation of Its Capsules

  • Received:2012-07-19 Revised:2012-10-25 Online:2012-12-15 Published:2012-12-15

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摘要：

为了探讨豆豉溶栓酶在小鼠体内的溶栓作用及其胶囊剂制备方法。本研究采用断尾法、玻片法和大鼠尾血栓模型法测定了枯草芽孢杆菌ND-43所产豆豉溶栓酶对小鼠体内凝血、出血时间及尾部血栓的影响。小鼠急毒实验表明：溶栓酶无毒副作用；连续灌胃给药1周，测定小鼠体内出血和凝血时间发现：高剂量80 (U/g?d)与中剂量组40 (U/g?d)能明显延长体内出血和凝血时间，抑制血液凝聚；大鼠尾血栓药效实验结果表明：高剂量组80 (U/g?d)对大鼠尾部血栓具有明显的溶栓效果，而中、低剂量组对大鼠尾血栓的溶栓效果不明显。此外，将其制成胶囊后各项指标均达到了胶囊制剂的相关要求。总之，溶栓酶具有较显著的溶栓作用而无明显的毒副作用，本实验所用胶囊制备工艺合理可行。

关键词: 京珠高速, 京珠高速

Abstract:

In order to research the fibrinolytic effect of fibrinolysis enzyme in vivo and the method to prepare its capsules, cutting tail procedure and glass slice method were carried out in this study. The results of acute toxicity test with mice showed that the Douchi Fibrinolytic Enzyme (DFE) was safe. After one week’s intragastric administration with mice by different doses of DFE, bleeding time and coagulation time in vivo were detected. The results showed that high 80 (U/g?d) and middle dose 40 (U/g?d) of DFE could extend the bleeding time and coagulation time obviously. The result of thrombolytic effect experiment in vivo suggested that the high dose (80 (U/g?d)) of DFE was resultful, but the effect of middle dose and low dose DFE were not obvious. In addition, the results indicated that the methods used in the study were fit to make the DFE into capsules. In short, the DFE, which was nontoxic, has great potential to be thrombolytic agents.

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