关键词: 中原农区, 中原农区

Abstract:

In order to determine and analyze the pathogeny of the clinical rabbits dying after natural infection, diagnosis was done to the rabbits by clinical symptoms, pathological changes and RT-PCR technology, as well as HA-HI test in this experiment. The results showed that the pathogen was rabbit hemorrhagic disease virus (RHDV). Then the complete sequence of capsid protein, VP60, which was the only structural protein of RHDV was amplified by RT-PCR with specific primers and amplification products were directly sequenced, assembled and analyzed. The results showed that the sequence of VP60 of RHDV isolated consisted of 1740 nucleotides just like other known strains. Phylogenetically, the sequence shared homology of more than 90% with worldwide isolates among RHDV strains and the highest homology of 98.1% with an isolate from Russia of 2009. From the study, we could saw that the virus isolated from untypical rabbit plague still had high conservation. However, there were also some minor variations in the structural protein, which was the basis of the classification of gene groups.