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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (16): 198-203.doi: 10.11924/j.issn.1000-6850.2012-3577

所属专题： 资源与环境； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

‘江华’苦茶资源生化成分的聚类分析研究

杨春 李端生 王庆 王汉超 陈涛林 罗军武   

  • 收稿日期:2012-11-02 修回日期:2012-11-23 出版日期:2013-06-05 发布日期:2013-06-05
  • 基金资助:
    《高茶黄素、高E-GCG单株选育》

Cluster Analysis of the Biochemical Composition in Camellia sinensis var. assamica ‘Jianghua’

  • Received:2012-11-02 Revised:2012-11-23 Online:2013-06-05 Published:2013-06-05

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544

摘要： 利用紫外分光光度计法和HPLC法测定江华苦茶群体品种单株茶树新梢中水浸出物、茶多酚、氨基酸、茶氨酸以及生物碱类和儿茶素类物质的含量。进一步对主要生化指标进行系统聚类分析，将100份单株分为6类，第I类表现为非酯型儿茶素含量最低；第II类表现为茶多酚、酚氨比、酯型儿茶素比例以及咖啡碱含量最低；第III类EGC含量最高；第IV类茶多酚、酚氨比以及可可碱含量最高，茶氨酸含量最少；第V类咖啡碱、D,L-C、EC、ECG、儿茶素总量、非酯型儿茶素的含量最高；第VI类氨基酸和茶氨酸含量最高，儿茶素总量最低。6个类群生化成分之间的差异明显，为优异茶树资源的选育提供帮助。

关键词: 长株潭地区, 长株潭地区

Abstract: Using UV spectrophotometer method and high performance liquid chromatography (HPLC) to analyze the biochemical component in fresh leaves of group varieties of Camellia sinensis var. assamica ‘Jianghua’. At the same time hierarchical cluster analysis showed that 100 individuals were divided into six categories. Class I performance of non-esterified catechins content was lowest. Class II polyphenols, phenolic ammonia ratio, the proportion of ester catechins and caffeine content were minimum. Class III EGC content was highest. Class IV polyphenols, phenolic ammonia ratio, theobromine were highest while theanine was at least. Class V caffeine, D,L-C, EC, ECG, catechins, non-ester type catechins content were highest. Class VI amino acid and theanine were highest and lowest total catechins. The obvious differences were existed in the six groups biochemical composition. It was helpful for the breeding of excellent tea tree resources.