欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (30): 173-180.doi: 10.11924/j.issn.1000-6850.2012-3727

• 生物技术科学 • 上一篇    下一篇

高DBT降解力脱硫菌株的脱硫条件优化

孙乐群 葛菁萍 王莉丽 平文祥   

  • 收稿日期:2012-11-16 修回日期:2013-04-16 出版日期:2013-10-25 发布日期:2013-10-25
  • 基金资助:
    国家自然科学基金

The Optimal Desulfurization Conditions of Desulfurization Strain with High Dibenzothiophene Degradation Ability

  • Received:2012-11-16 Revised:2013-04-16 Online:2013-10-25 Published:2013-10-25

PDF (PC)

584

摘要： 本课题是从大庆油田土壤中分离得到一株专一性脱硫菌株HDBS-1，经鉴定，该菌种属为坂崎肠杆菌（Enterobacter sakazakii），通过诱变筛选得到了一株脱硫能力最高的菌株HDBS-4。该微生物可以按照特异性脱硫途径将DBT转化为2-HBP（2-羟基联苯）。为提高脱硫力，本试验对HDBS-4的脱硫条件及培养基进行了优化。最终确定20mL/250mL为装液量，按6%接种量， 转速为300r/min，于30℃连续培养5d。当在上述最优脱硫条件下培养时，发酵液中2-HBP生成量可达6.1105mg/L，较优化前提高了2倍多，较原始菌株提高了12倍多。

关键词: 乳蛋白, 乳蛋白

Abstract: Abstract: A desulfurization strain HDBS-1 was selected which can degrade dibenzothiophene (DBT)，which was identified as Enterobacter sakazakii. This strain can convert dibenzothiophene to 2-hydroxybiphenyl (2-HBP) through specific desulfurization pathway. The best desulfurization conditions have been researched for getting a higher ability Desulfurization strain .w e find the best optimum conditions are that the cultures containing 25mL in every 250mL medium, 6% inoculated, 30℃ in temperature.In this optimum medium, the obtained strain HDBS-4 raised its ability to convert dibenzothiophene to 2-hydroxybiphenyl (2-HBP) , which gave a fold increase compared with usual condition.Even 12 times higher than the ability of the original strain.