Abstract: Study on the changes of metabolites during the stage of planting to flower bud differentiation was conducted using cultivars of ‘Asiatic’ and ‘Oriental’ hybrids. The results showed that the processes of metabolism of soluble sugar and soluble protein and the activity of POD were positively correlated with that of flower bud differentiation. The intensities of metabolism of the soluble sugar and soluble protein and the activity of POD in ‘Yellow Baby’ were higher than that in ‘Casa Blanca’. The process of flower bud differentiation of ‘Yellow Baby’ is faster than that of ‘Casa Blanca’. Starch is unnecessary during flower bud differentiation of lilies.