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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (26): 25-30.doi: 10.11924/j.issn.1000-6850.2013-0115

所属专题： 水产渔业

• 水产 渔业 • 上一篇    下一篇

甲砜霉素在鲤鱼及鲫鱼体内的残留研究

韩冰 杨洪波 王荻 卢彤岩   

  • 收稿日期:2013-01-11 修回日期:2013-02-20 出版日期:2013-09-15 发布日期:2013-09-15
  • 基金资助:
    行业专项;十二五支撑计划

Study on Thiamphenicol Residue in Common Carp and Crucian Carp

  • Received:2013-01-11 Revised:2013-02-20 Online:2013-09-15 Published:2013-09-15

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摘要： 本研究旨在分析甲砜霉素在鲤鱼和鲫鱼体内的残留消除规律并为制定休药期提供依据。按照30 mg/kg的给药量对鲤鱼和鲫鱼连续口灌给药甲砜霉素3天，定时取样，外标法定量，采用高效液相色谱-串联质谱法(HPLC-MS/MS)检测采样组织中甲砜霉素的残留量。结果显示：甲砜霉素在0.1~200 ng/mL范围内有良好的线性关系，相关系数为0.9987。回收率在81.33%~109.72%之间。甲砜霉素在停止给药后鱼体各检测组织中残留量呈下降趋势，在停药25天后，甲砜霉素残留量低于中国与欧盟规定的最高残留限量(MRLs)。根据结果，建议休药期为25天。

关键词: 优化选择, 优化选择

Abstract: This experiment was conducted to study the elimination of Thiamphenicol (TAP) residue in common carp (cyprinus carpio) and crucian carp (carassius auratus), preparing for the determination of the withdrawal time. After oral administration of TAP with a dosage of 30 mg/kg body weight per day for 3 days, the fish was sampled at certain time. Quantitative analysis was performed with external standard method and TAP concentrations in tissues were analyzed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The results showed that the linear relationship of TAP was nice (r2=0.9987) in 0.1 ng/mL to 200 ng/mL concentration range and the recovery ranged from 81.33% to 109.72%. The detection indicated that the residues of TAP in common carp and crucian carp had a down trend. At the 25th day, the residue started to below the maximum residue limits (MRLs) of China and EU. It was proposed that the withdrawal time should be 25 days after administration.

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