Abstract: Effects of long-term fertilization on accumulating and releasing of phosphorus in greenhouse soil was investigated. The results showed: Total phosphorus, rapidly available phosphorus and organic phosphorus in soil fertilized with phosphate fertilizer present the obviously higher content than that in un-fertilized soil. Moreover, the content of phosphorus with different species can be increased by fertilizing organic manure under the same condition. The content of total phosphorus in the four kinds of treatments present the orders: AP>BP>A>B, the orders of rapidly available phosphorus and organic phosphorus are: AP > A > BP > B. The organic manure can stimulate the releasing of soil phosphorus apparently. The orders of accumulating-releasing capacity of soil phosphorus in the four different kinds of soil is: AP>BP>A>B. The releasing characteristic of soil phosphorus can be exactly depicted by parabolic diffusion equation.