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中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (7): 33-33.

所属专题： 生物技术

• 目次 • 上一篇    下一篇

蚊净香草组织培养技术研究

李伟明,林 纬,黎起秦,陶 劲,蓝桃菊   

  • 出版日期:2005-07-05 发布日期:2005-07-05

The Tissue Culture Studies of Mozzie buster

Li Weiming, Lin Wei, Li Qiqin, Tao Jing, Lan Taoju   

  • Online:2005-07-05 Published:2005-07-05

摘要： 以蚊净香草的叶片和茎段作为外植体，用0.1%的升汞作为消毒剂，通过试验拟找出最佳的消毒时间及适于外植体的诱导、增殖和生根的培养基。结果表明：0.1%的升汞对两种外植体的最佳消毒时间是5~7min，最适于诱导外植体产生愈伤组织和不定芽，以及不定芽的增殖和生根的培养基分别为：MS+6-BA0.5mg/L(单位下同)+IAA0.1+NAA0.3、MS+6-BA0.25 +IAA0.5、 MS+6-BA0.05+NAA0.5 +IBA0.5。

Abstract: The tissue culture and rapid propagation of Mozzie buster were studied by using tips and leaves as explants.The results showed that: The optimum disinfect time for the explants with Hgcl2 was 5~7 min.The explants cultured on MS+6-BA0.5mg/L+IAA0.1mg/L+NAA0.3mg/L ,and MS+6-BA 0.25mg/L+IAA0.5mg/L, and MS+6-BA0.05mg/L+NAA0.5mg/L+IBA0.5 mg/L were suitable for inducing buds,prolifetating buds and rooting of microshoots,respectively.