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中国农学通报 ›› 2013, Vol. 29 ›› Issue (34): 71-75.doi: 10.11924/j.issn.1000-6850.2013-1207

所属专题: 烟草种植与生产

• 林学 园艺 园林 • 上一篇    下一篇

不同耐性烟草品种苗期叶片光合特性对低温胁迫的响应

胡日生 曾中 戴杏华 杨全柳 贺利雄 刘志   

  • 收稿日期:2013-04-26 修回日期:2013-05-23 出版日期:2013-12-05 发布日期:2013-12-05
  • 基金资助:
    中国烟草总公司科技重点项目;中国烟草总公司湖南省公司科技项目

Response of Leaf Photosynthetic Characteristics of Two Different Tolerant Tobacco Cultivars at Seedling Stage to Low Temperature Stress

  • Received:2013-04-26 Revised:2013-05-23 Online:2013-12-05 Published:2013-12-05

摘要: 对不同耐性烟草苗期叶片的光合特性在低温胁迫及其随后常温恢复生长条件下的变化进行了详细研究。结果表明,4℃~6℃的低温胁迫几乎完全抑制较耐冷烟草品种K326和敏感品种云烟97幼苗的生长,但在恢复至常温条件时K326的生长速度显著高于云烟97。低温胁迫降低烟草幼苗叶片的光合作用,而且胁迫时间越长,抑制效应越明显,处理6d后叶片气体交换参数净光合速率(Pn)、气孔导度(Gs) 、胞间CO2浓度(Ci) 、蒸腾速率(Tr)、水分利用效率(WUE)、气孔限制值(Ls)以及叶绿素荧光参数最大光化学效率(Fv/Fm)、PS II量子产量或光能捕获的效率(ΦPSII)、光化学猝灭系数(qP)、表观电子传导率(ETR)和非光化学猝灭系数(NPQ)均与处理前的差异达到了显著水平。恢复至常温生长条件时,幼苗叶片光合作用迅速得以增强,K326提高的幅度比云烟97大;虽然恢复至第9d时这两个品种的光合作用还没有回复到处理前的水平,但较耐冷品种K326的光合作用参数与处理前的差异较小,而低温敏感品种云烟97与处理前仍存在显著性差异。

关键词: 可用性, 可用性

Abstract: The changes in leaf photosynthetic characteristics of two different tolerant tobacco cultivars at seedling stage under low temperature stress and subsequent room temperature condition were investigated in the paper. The results showed that the seedling growth of the moderate tolerant tobacco cultivar K326 and the sensitive one Yunyan 97 was nearly inhibited completely at low temperature stress from 4℃ to 6℃, while the growth rate of K326 was significantly higher than that of Yunyan 97 under subsequent room temperature. The leaf photosynthesis at seedling was reduced under low temperature stress, and the inhibitory effects became worse with the elongation of stress treatment. The leaf photosynthetic indexes such as net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci), transpiration rate (Tr), water use efficiency (WUE), stomatal limitation (Ls), maximum photochemical efficiency of photosystem II (Fv/Fm), quantum yield of electron transport (ΦPSII), photochemical quenching co-efficient (qP), apparent electron transfer rate (ETR) and non-photochemical quenching co-efficient (NPQ) under low temperature for 6 days were significantly different with the untreated control. The leaf photosynthesis of tobacco seedlings was raised under subsequent room temperature condition, and the increasing range in K326 was much more than that of Yunyan 97. Although the leaf photosynthesis of the both two cultivars under subsequent room temperature for 9 days was not restored to the levels before low temperature stress, the leaf photosynthetic indexes in the moderate tolerant cultivar K326 were only a little lower than untreated seedlings, while these indexes in the sensitive cultivar Yunyan 97 were still significantly different with the untreated ones.