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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (28): 105-109.doi: 10.11924/j.issn.1000-6850.2013-1448

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

油木奈果实β-tubulin cDNA的克隆及表达

王蓓 郭志雄 钟凤林 潘腾飞   

  • 收稿日期:2013-05-22 修回日期:2013-07-23 出版日期:2013-10-05 发布日期:2013-10-05
  • 基金资助:
    福建省青年人才项目

cDNA cloning and expression of β-tubulin in plum (Prunus salicina Lindl var. cordata JY Zhang et al) fruit

  • Received:2013-05-22 Revised:2013-07-23 Online:2013-10-05 Published:2013-10-05

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摘要： 以油木奈 果实为材料，应用RT-PCR和RACE技术对其β-tubulin cDNA进行了克隆，结果表明，油木奈果实β-tubulin的cDNA全长1606bp，包含一个1341bp的开放阅读框，共编码446个氨基酸。对其推导氨基酸序列进行比对分析，表明油木奈 果实β-tubulin与众多植物来源的β-tubulin具有很高的相似度。通过设计特异性引物，采用半定量RT-PCR的方法对果实不同发育β-tubulin表达差异进行了初步分析，结果表明，该β-tubulin基因表达量稳定，为进一步应用实时荧光定量PCR技术研究油木奈 果实发育相关基因的表达奠定了试验基础。

关键词: 重庆永川, 重庆永川

Abstract: A full-length cDNA of β-tubulin was obtained from plum (Prunus salicina Lindl var. cordata JY Zhang et al cv. Younai) fruit by means of RT-PCR and RACE. The complete cDNA was 1606bp in length, contained an opening reading frame of 1341bp encoding a peptide of 446 amino acid residues. The deduced amino acid sequence has high identity to those of many other plants. Specific primers were designed, semi-quantitative RT-PCR was performed, and the results showed that the expression of this gene was constant at 11 of different stages of fruit development, suggesting its availability for reference gene in the subsequent investigation on the expression of development-related genes in plum fruit using real-time quantitative PCR.

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