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中国农学通报 ›› 2005, Vol. 21 ›› Issue (8): 256-256.

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大花蕙兰种子的液体保藏研究

姚献华,王利民,   

  • 出版日期:2005-08-05 发布日期:2005-08-05

Studies on Liquid Storage of Seeds of Cymbidium hybridium

Yao Xianhua, Wang Limin,   

  • Online:2005-08-05 Published:2005-08-05

摘要: 大花蕙兰是一种具有极高观赏价值的兰属植物,其主要的育种方法是杂交育种。杂交后得到的种子很多,每个果实中有种子数万粒,需进行无菌播种才可以萌发。即便在低温下,种子在空气的保藏时间也很短。把种子放在按照MS培养基配方配制的液体中,PH5.4,蔗糖30g/L,每毫升保藏液中保藏种子数量在50粒以上,环境温度控制在15℃左右,光照约2000lux,保藏液每天早晚震荡两次,每次2h,即可获得理想的保藏效果。

Abstract: Cymbidium hybridium is a very important ornamental plant. Hybridization is a primary method of breeding of Cymbidium hybridum. There are ten thousands of seeds in one fruit .The seeds must be sterile seeded. They have short lives in air even though the temperature is low . The seeds are putted in liquid MS medium .PH 5.4, sugar 30g/L ,quantity of seeds per milliliter above 50 granules ,temperature about 15℃, light illumination about 2000lux .Storage liquid is twice vibrated everyday. Every time is two hours .The effect of storage is perfect.