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中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (8): 299-299.

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用RAPD标记研究多花水仙若干品种类型的亲缘关系

吴菁华,吕柳新,张志忠   

  • 出版日期:2005-08-05 发布日期:2005-08-05

A Study on Genetic Relationship among Several Narcissus Cultivars by RAPD Markers

Wu Jinghua, Lu Liuxin, Zhang Zhizhong   

  • Online:2005-08-05 Published:2005-08-05

摘要： 利用随机引物多态性DNA（RAPD）技术分析了7个多花水仙品种类型，从260条随机引物中筛选出18条，共扩增出129条稳定条带，以此来计算品种类型间遗传相似系数，进行聚类分析，构建树状分枝图。结果表明：7个品种类型可分成四组：第一组I-1和I-2；第二组II-1和 II-2；第三组III-1和 III-2；III-3单独成为一组。

Abstract: Seven cultivars were analyzed, using random amplified polymorphic DNA(RAPD) genetic markers. Eighteen decanuletide primers were used for DNA amplification, 129 markers generating. The genetic marker data were analyzed by using NT-sys software. A dendrogram was constructed by using UPGMA method. The study showed that it was similar between I-1 and I-2.II-1 and II-2 were similar. It also indicates that III-1and III-2 have a close relationship. III-3 is a special specie.