真菌除草剂的研究进展

摘要/Abstract

摘要： 真菌除草剂具有防治杂草的潜在优势。目前具有生物潜能的真菌主要集中在盘苞菌属等9个属中，国内外已筛选出对多种杂草具有良好除草活性的真菌菌株，部分菌株防治效果可与化学除草剂相媲美；但其寄主专一性高、发酵与制剂加工困难等限制了其在生产上的广泛应用。不同真菌间的混用、真菌与低剂量化学药剂间的混用及可克服其限制因素，提高病菌致病力；此外，制剂加工方法的改进，病原微生物与昆虫制品的综合利用，特别是转基因技术和DNA重组技术在菌株改良上的应用成为真菌除草剂的热点研究方向。

Abstract: Using mycoherbicides for control of weeds has great potential. The fungi with herbicidal acitivity are mostly focus on the fungus colletotrichum and other eight fungi.Now it has screened many herbicidal activity strains in the world. Control efficient of some strains are as well as chemical herbicides. But the difficulty of fermentation and formulation, the high host-specifity and other problems are limit its application in production. The application of mycoherbicide combine with low rates of herbicides or other fungi can overcome some limitations, improve the pathogenicity. In addition, the improvement of formulation, comprehensive utilization of pathogenic microbes and insect agents, especially strain improving by transgenic technology and DNA recombination technology have become hot research directions.

肖艳松,柏连阳,陈桂华. 真菌除草剂的研究进展[J]. 中国农学通报, 2005, 21(8): 345-345.

Xiao Yansong, Bai Lianyang, Chen Guihua. Current Advances in Mycoherbicides Research[J]. Chinese Agricultural Science Bulletin, 2005, 21(8): 345-345.

参考文献

1 张玉聚,孙化田,王春生.除草剂及其混用与农田杂草化学防治[M].北京:中国农业出版社,2000.3~6
2 李扬汉,张宗俭,王建书,等.有关真菌除草剂研究的进展[J].生物防治通报,1994,10(1):35~39
3 顾成波,赵长山.微生物及其天然产物防治杂草的发展及展望[J].农药科学与管理,2003,24(2):19~21
4 朱秦,强胜.真菌除草剂及其大批量生产工艺研究现状[J].杂草科学,2002,(4):1~4
5 陈勇,倪汉文.中国稗草病原真菌对稗草及水稻的致病性[J].中国生物防治,1999,15(2)73~76
6 陈勇,倪汉文.尖角突脐孢对稻田稗草的防除效果[J].植物保护学报,2001,28(1)73~76
7 曾青,强胜.波斯婆婆纳生防菌株QZ-97a的分离鉴定与致病性研究[J].南京农业大学学报,2000,23(3):21~24
8 强胜.胶孢炭疽菌波斯婆婆纳专化型及其侵染过程[J].植物保护学报,2001,28(3):279~284
9 向梅梅,曾永三,刘任,等.莲子草假隔链格孢的寄主范围及对空心莲子草的控制作用[J].植物病理学报.2002,32(3):286~287
10 Peng G, Byer K N, Bailey K L. Pyricularia setariae: a potential bioherbicide agent for control of greenfoxtail (Setaria viridis). Weed Science[J], 2004,52(1):105~114
11 Thomas H, Sauerborn J, Muller-Stover D, et al. Fusarium oxysporum f. sp. orthoceras, a potential mycoherbicide, parasitizesseeds of Orobanche Cumana (sunflower broomrape): A cytological study[J]. Annals of Botany, 1999,83(4):453~458
12 Muller-Stover D, Kroschel J, Thomas H, et al. Chlamydospores of Fusarium oxysporum Schlecht f.sp orthoceras (Appel &Wollenw.) Bilai as inoculum for wheat-flour-kaolin granules to be used for the biological control of Orobanche cumana Wallr[J]. European journal of plant pathology, 2002,108(3):221~228
13 Kadir J B, Charudattan R, Stall W M, et al. Field efficacy of Dactylaria higginsii as a bioherbicide for the control ofpurple nutsedge (Cyperus rotundus)[J]. Weed technology, 2000,14(1):1~6
14 Gupta V P, Kumar V, Mishra R K, et al. Puccinia romagnoliana Marie&Sacc.-a potential bioherbicide agent for biocontrol of purple nutsedge(Cyperus rotundus L.) in mulberry[J].Journal of Phytopathology. 2002,150(4~5):263~270
15 Yang YongKook, Kim SoonOk, Chung HooSup, et al. Use of Colletotrichum graminicola KA001 to control barnyard grass[J]. Plant Disease, 2000,84(1):55~59
16 Becker E M, Hintz, et al. PCR-based genetic markers for detection and infection frequency analysis ofthe biocontrol fungus Chondrostereum purpureum on Sitka alder and trembling aspen[J]. Biological Control, 1999,15(1):71~80
17 Prasad R, Kushwaha S. Ecological-based Weed Management for the 21st century:Biological Control of Forest Weeds by Mycoherbicide Agent,Chondrostereum purpureum. 18th Asian-Pacific Weed Science Society Conference. May 28-Jun 2,2001 Beijing,P.R.China, 2001:348~352
18 Paul Y. dela Bastide, Hong Zhu, et al. Chondrostereum purpureum: An Alternative to Chemical Herbicide Brush Control. The seventh International Sym posium on Environmental Concerns in Rights-of- Way Management Sep9-13,2000 Calgary,Alberta, Canada, 2002:120~124
19 Netland J, Dutton L C, Greaves M P, et al. Biological control of Chenopodium album L. in Europe[J]. BioControl, 2001,46(2):175~196
20 Grace B S, Muller-Scharer H. Biological control of Senecio vulgaris in carrots(Daucus carota) with the rust fungus Puccinia lagenophorae[J]. Basic and Applied Ecology, 2003,4(4):375~384
21 El-Sayed W, Kang Z, Hurle K. Infection process of Phomopsis convolvulus as a mycoherbicide for Convolvulus arvensis L.Zeitschrift fur Pflanzenkrankheiten und Pflanzenaschutz[J], 2002,109(2):180~192
22 Saxena S, Pandey A K. Evaluation of an indigenous isolate of Alternaria alternata (LC#508) for use as a mycoherbicide for Lantana camara L.[J]. Crop Protection, 2002,21(1):71~73
23 Zhang W M, Sulz M, Myketiak T. Potential of using microbes and microbial natural products for control of weeds[C]. 18th Asian-Pacific Weed Science Society Conference. May 28-Jun 2, 2001 Beijing, P. R. China, 2001:358~365
24 付颖,叶非.生物源除草剂研究与使用进展[J].农药,2002,4l(5):7~10,17
25 邢来君.真菌除草剂的研究现状与展望[J].真菌学报,1995,14(2):153~157
26 杨文权,慕小倩,安德荣.微生物除草剂开发的限制因素和对策[J].农药,2003,42(8):8~9
27 曾青,强胜.低剂量化学除草剂对菌株Qz-97a侵染波斯婆婆纳的影响[J].植物保护学报,2002,29(4)361~365
28 Amsellem Z, Cohen B A, Gressel J. Engineering hypervirulence in a mycoherbicidal fungus for efficient weed control[J]. Nature biotechnology. 2002,20(10):1035~1039
29 Zhang W M, Wolf T M, Bailey K L, et al. Screening of adjuvants for bioherbicide formulations with Colletotrichum spp. and Phoma spp[J]. Biological Control, 2003,26(2):95~108
30 Winder R S., Wheeler J J, Conder N, et al. Microencapsulation: a strategy for formulation ofinoculum[J]. Biocontrol Science and Techno logy. 2003,13(2):155~169
31 黄世文,余柳青.影响链格孢菌生长及产孢因子[J].中国生物防治,2001,17(1):16~19
32 强胜,郭爱民,李扬汉,等.大批量生产百日草链格孢菌孢子的技术[J].中国生物防治,1997,13(4):169~172
33 Abbas H K, Boyette C D. Solid substrate formulations of the mycoherbicide Colletotrichum truncatum for hemp sesbania(Sesbania exaltata) control[J]. Biocontrol Science and Technology, 2000,10(3):291~300
34 李水清,赵春.我国生物除草剂的研究进展[J].湖北农学院学报,2003,23(2):135~139
35 Kluth S, Kruess A, Tscharntke T. Influence of mechanical cutting and pathogen application on the performance and nutrient storage of Cirsium arvense[J]. The Journal of Applied Ecology, 2003,40(2):334~343
36 罗小刚.微生物除草剂的发展现状与展望[J].甘肃科学学报,1995,7(3):54~60
37 刘焕绿,刘亦学,刘晓林,等.微生物除草剂研究概况与建议[J].天津农学院学报,2002,7(4):36~39