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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (28): 295-300.doi: 10.11924/j.issn.1000-6850.2014-1187

所属专题： 生物技术

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1株枯草芽孢杆菌的分离鉴定及其发酵条件优化

鞠瑞成,王鹏,公春艳,赵宏涛,刘露,李园园   

  1. 青岛明月蓝海生物科技有限公司,,青岛明月蓝海生物科技有限公司,青岛明月蓝海生物科技有限公司,青岛明月蓝海生物科技有限公司,青岛明月海藻集团有限公司
  • 收稿日期:2014-04-23 修回日期:2014-04-23 接受日期:2014-08-26 出版日期:2014-10-15 发布日期:2014-10-15
  • 通讯作者: 鞠瑞成
  • 基金资助:
    海洋经济创新发展区域示范项目 “绿色农用生物产品多功能海藻肥项目”。

Isolation, Identification and Fermentation Optimization of An Antibiotic Bacillus subtilis

  • Received:2014-04-23 Revised:2014-04-23 Accepted:2014-08-26 Online:2014-10-15 Published:2014-10-15

摘要： 为筛选出对黄瓜枯萎病具有生防作用的拮抗菌，利用平板对峙法从黄瓜根际土壤中分离获得1株对黄瓜枯萎病的致病菌尖孢镰刀菌具有较强抑制作用的细菌，通过形态特征、生理生化特征及16SrRNA序列分析，将该菌株鉴定为枯草芽孢杆菌(Bacillus subtilis)，并对其发酵条件进行了优化，获得的最佳发酵条件：种龄10 h，接种量4%，发酵温度32℃，装液量50 mL/250 mL，发酵转速180 r/min，发酵时间36 h，采用优化后的发酵条件，发酵液菌落数比初始值提高了172%。

关键词: 地理变异, 地理变异

Abstract: In order to screen strains of antagonistic bacteria against cucumber fusarium wilt, one bacteria strain was isolated from the rhizosphere soil of cucumber by the methods of plate confrontation, and the inhibitive efficiency on the hyphal growth of Fusarium oxysporum f. sp. cucumeris was tested. The results showed that the antagonistic bacteria strains Th-1 had significant inhibition effect on Fusarium oxysporum f. sp. cucumeris. By the homology analysis of 16S rRNA sequence together with morphological, physical and biochemical characteristics, Th- 1was identified as Bacillus subtilis and its fermentation conditions was optimized. The optimal fermentation conditions were composed of the following factors: seed age, 10 h; inoculum, 4% (v/v); fermentation temperature, 32?C; liquid volume, 50 mL/250 mL; rotation speed, 180 r/min; incubation time, 36 h. This optimization strategy enhanced the fermentation liquid colonies nearly 1.72 times of the initial value.