欢迎访问《中国农学通报》,

中国农学通报 ›› 2014, Vol. 30 ›› Issue (30): 269-272.doi: 10.11924/j.issn.1000-6850.2014-1407

• 生物技术科学 • 上一篇    下一篇

近红外光谱技术与常规方法检测紫花苜蓿品质的对比研究

刘温   

  1. 中国农业大学动物科技学院草业科学系
  • 收稿日期:2014-05-15 修回日期:2014-05-15 接受日期:2014-08-18 出版日期:2014-10-31 发布日期:2014-10-31
  • 通讯作者: 刘温
  • 基金资助:
    国家级本科生项目——科研创新项目“牧草品质快速评价规范研究”(201310019034)

Comparative Study on Quality of Alfalfa with Near-Infrared-Reflectance Spectroscopy and Conventional Chemical Assay

  • Received:2014-05-15 Revised:2014-05-15 Accepted:2014-08-18 Online:2014-10-31 Published:2014-10-31

摘要: 从黑龙江省国家牧草产业技术体系绥化综合试验站的试验田中采集的10 种(每种取7 个样品)紫花苜蓿样品分别采用近红外光谱技术和常规化学测定法,对其含有的粗蛋白(CP)、中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)、脂肪(FAT)、灰分(ASH)、干物质(DM)成分进行了测定,并将测试结果用SPSS 软件进行配对T值分析比较,结果表明:两种方法的X ± S 相近,|t|<0.05,P>0.05,差异不显著。分别计算两种方法的标准偏差(S)和相对标准偏差(RSD),发现近红外分析方法的值均小于常规化学分析方法。试验结果表明:基于与常规化学方法的比较,近红外方法可以作为一种新的检测方法被使用,值得广泛应用于各种牧草饲养品质的测定与推广。

关键词: 休药期, 休药期

Abstract: Collecting 10 kinds of alfalfa samples from national technical system of grass industry in Suihua general field, we measured some indexes of alfalfa with near-infrared-reflectance Spectroscopy (NIRS) and conventional chemical assay (CCA). The crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), fat (FAT), ash (ASH) and dry matter (DM) were detected. Compared with paired T value analysis by SPSS, the results show that X ± S are approximate between two methods, |t|<0.05, P>0.05. Calculating the standard deviation (SD) and the relative standard deviation (RSD) respectively, we find that the value of NIRS is less than CCA. The results also show that NIRS can be used as a new detection method, based on the comparison with CCA.