猪卵母细胞冷冻损伤的研究进展

摘要/Abstract

摘要： 卵母细胞冷冻保存技术的发展为体外受精、核移植以及转基因动物生产等胚胎工程技术提供了极大的灵活性，同时为家畜保种及珍稀濒危动物的保护提供了新的技术途径。在此，综述了以猪为主的家畜卵母细胞的冷冻保存的原理及冷冻保存对卵母细胞超微结构和发育潜力的影响，以期为猪卵母细胞的冷冻保存研究提供一定的理论参考，从而提高猪卵母细胞冷冻后的成活率和体外受精生产效率。

Abstract: The development of oocytes cryopreservation would generate a convenient and valued source of oocytes for such procedures as in vitro fertilization, cloning, transgenic and other releated embryo engineering technology. At the same time, it also provided a technique base for the conservation of super animal breed and endanged wildlife species. Cryopreservation of oocytes was not as effective as that of embryo. This review mainly deals with the principle of cryopreservation, effect of cryopreservation on the structure and developmental competence of porcine oocytes.This paper can also provide some advices for IVF and IVM on porcine.

李健,胡建宏,韩增胜,,杨海,,李青旺,. 猪卵母细胞冷冻损伤的研究进展[J]. 中国农学通报, 2005, 21(10): 21-21.

Li Jian, Hu Jianhong, , Han Zengsheng, ,Yang Hai, , Li Qingwang,. Progress on Cryopreservation Damage of Porcine Oocytes[J]. Chinese Agricultural Science Bulletin, 2005, 21(10): 21-21.

参考文献

1 Whittingham D G. Fertilization and development to term of unfertilized mouse oocytes previously stored at -196℃[J]. J Reprod Fert 1977,49:89~94
2 Isachenko V, Soler C, Isachenko E, et al. Vitrification of immature porcine oocytes: Effects of lipid droplets,temperture,cytoskeleton and addition and removel of cryoprotectant[J]. Cryobiology,1998,36:250~253
3 Vajta G, Holm P, Kuwayama M, et al. Open pulled Straw(OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos[J]. Mol Reprod Dev,1998,51:53~58
4 Friedler S,Giudice LC,Lamb E J. Cryopreservation of embryo and ova[J]. Fertil steril,1988,49:743~764
5 冯怀亮.哺乳动物胚胎工程[M].长春:吉林科学技术出版社,1994.147~149
6 刘海军,张美佳,侯蓉,等.OPS玻璃化冷冻对山羊卵母细胞超微结构的影响[J].西北农林科技大学学报（自然科学版）,2001,29(3):9~12
7 王光亚,段恩奎.山羊胚胎工程[M].陕西杨凌:天则出版社,1994.61~62
8 Aman R R, Parks J E. Effects of cooling and rewarming on the meiotic spindle and chromosome of in vitro-matured bovine oocytes[J]. Biol Reprod,1996,54:1059~1069
9 Wininger J D, Kort H I. Cryopreservation of immature and mature human oocyte[J]. Semin Reprod Med,2002,20:45~50
10 Vincent C, Johnson M H. Cooling cryoprotectants and the cytoskeleton of the mammalian oocyte[J].Oxford Rev. Reprod Biol,1992,14:73~100
11 Saunders K M, Parks J E. Effects of cryopreservation procedure on the cytology and fertilization rate if in vitro-matured bovine oocytes[J]. Biol Reprod,1999,61:178~187
12 冯怀亮,孙青原,李子义.牛体外成熟卵母细胞冷冻保存的研究[J].畜牧兽医学报,1995,26(6):481~486
13 孙青原,秦鹏春,刘国艺,等.牛卵泡卵母细胞冷冻损伤的研究[J].中国兽医学报,1994,14(1):41~47
14 Fuku E, Xia L, Downey B R. Ultrastructural changes in bovine oocytes cryopreservated by vitrification[J]. Cryobiology, 1995,32:139~156
15 Hochi S, Kozawa M, Fujimoto T. In vitro maturation and transmission electron microscopic of observation of horse oocyte after vitrification[J]. Cryobiology,1996,33:300~310
16 Bredkjaer HE, Grudzinskas JG. Cryobiology in human assisted reproductive technology, would Hippocrates approve[J]? Early Pregnancy,2001,5:211~213
17 Chia C M, Chan W B, Quah E, et al. Triploid pregnancy after ICSI of Frozen testicular spermatozoa into cryopreserved human oocytes: case report[J]. Hum Reprod,2000,15:1962~1964
18 Sounders K M, Parks J E. Effects of cryopreservation procedure on the cytology and fertilization rate if in vitro-matured bovine oocytes[J]. Biol Reprod,1999,61:178~187
19 Niemann H. Cryopreservation of ova and embryos from livestock:current status and research needs[J]. Theriogenology,1991,35:109~124
20 朱捷,张忠诚,刘译泉.猪卵母细胞在玻璃化冷冻液中的培养效果[J].中国畜牧杂志,1995,31(5):7~8
21 Nagasbima H, Kuwayama M, Grupen C G, et al. Vitrification of porcine early cleavage stage embryos and oocytes after removal of cytoplasmic lipid droplets[J]. Theriogenology,1996,45:180(Abstr)
22 Volodimir Isachenko, Carles Soler, Eugenia Isachnko, et al. Vitrification of immature porcine oocytes:effects of lipid droplets, temperature, cytoskeleton and addition and removal of cryoprotectant[J]. Cryobiology,1998,36:250~253
23 蔡令波,王锋.猪卵母细胞体外成熟与冷冻保存的初步研究[J].南京农业大学学报,2002,25(4):116~118