欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (1): 127-131.doi: 10.11924/j.issn.1000-6850.2014-2020

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

生长调节剂诱导霍山石斛试管开花研究

李 杰,章金辉,朱根发,王再花   

  1. (广东省农业科学院环境园艺研究所/广东省园林花卉种质创新综合利用重点实验室,广州 510640）
  • 收稿日期:2014-07-23 修回日期:2015-01-07 接受日期:2014-11-24 出版日期:2015-03-18 发布日期:2015-03-18
  • 通讯作者: 李杰
  • 基金资助:

    基金项目：“十二五”农村领域国家科技计划课题“热带兰种业关键技术研究”(2012BAD01B0702)；广东省科技厅农业领域引导项目“两种濒危石斛兰试管开花研究”；广东省科技计划项目“兰花生产技术推广与培训”(2012B040400008)。

Effect of Growth Regulators on Flowering in Vitro of Dendrobium huoshanense

Li Jie, Zhang Jinhui, Zhu Genfa, Wang Zaihua   

  1. (Environmental Horticulture Research Institute of Guangdong Academy of Agricultural Sciences/Guangdong Key Lab of Ornamental Plant Germplasm Innovation and Utilization, Guangzhou 510640)
  • Received:2014-07-23 Revised:2015-01-07 Accepted:2014-11-24 Online:2015-03-18 Published:2015-03-18

摘要：

为筛选合适的离体诱导霍山石斛开花的激素配比，以3个月大的霍山石斛无菌幼苗为材料，比较6-BA、2,4-D、NAA、Ad、PP333和TDZ 6种生长调节剂组合对霍山石斛试管开花的影响。结果表明，不同生长调节剂组合均能显著促进霍山石斛花芽形成，其中0.5 mg/L 6-BA+ 0.2 mg/L 2,4-D处理效果最佳，120天内花芽诱导率、开花率和正常花比率分别达35%、20%和8.9%，而0.5 mg/L 6-BA+0.2 mg/L NAA处理次之，花芽诱导率、开花率和正常花比率分别达24.4%和12.8%和5.0%。

关键词: 关键评价指标, 关键评价指标

Abstract: In order to obtain the appropriate growth regulators for flowering in vitro of Dendrobium huoshanense, laboratory experiments were conducted to investigate the collaboration effects of growth regulators (6-BA, 2,4-D, NAA, Ad, PP333 and TDZ) on the flowering in vitro of D. huoshanense. The results showed that growth regulator combination increased significantly the flower bud of D. huoshanense, and the optimum treatment was 0.5 mg/L 6-BA 0.2 mg/L 2,4-D, with the 35% flower bud induction rate, 20% flower rate and 8.9% normal flowers in 120 days. The next best treatment was 0.5 mg/L 6-BA 0.2 mg/L NAA, with the 24.4% flower bud induction rate, 12.8% flower rate and 5.0% normal flowers.