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中国农学通报

中国农学通报 ›› 2004, Vol. 20 ›› Issue (2): 1-1.

• 目次 •    下一篇

光温条件及碳源对苎麻愈伤生长和分化的影响

王晓玲 彭定祥   

  • 出版日期:2004-04-05 发布日期:2004-04-05

Effects on callus induction and differentiation of different light time, temperature and type of carbohydrate source from Ramie.

Wang Xiaoling Peng Dingxiang   

  • Online:2004-04-05 Published:2004-04-05

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636

摘要： 本试验用湘苎2号为材料，研究了不同光照时数和碳源、不同温度和碳源对苎麻愈伤生长和分化的影响，方差分析结果表明，用葡萄糖作碳源比蔗糖更有利于苎麻愈伤生长；12h光照/12h黑暗最有利于苎麻愈伤的生长，其次是全光照条件，黑暗条件下愈伤生长最慢。用葡萄糖作碳源时，愈伤生长量在30℃、28℃、25℃条件下均极显著大于23℃条件下；蔗糖作碳源时，在25～28℃的温度范围内有利于愈伤组织的增殖，而过高或者过低都是不利的。光照处理对分化是有利的，而相对较高温条件虽然能比较好的促进愈伤增殖，但这种来源的愈伤组织分化再生不如相对较低的温度条件下生长的愈伤。

Abstract: With Ramie variety of Xiangzhu No.2 as research material, this test studied effects on callus induction and differentiation of different light time, temperature and type of carbohydrate source from Ramie. The results of variance analysis indicated that culture media of glucose as carbohydrate source is superior to of sucrose in induction callus; 12h light/12h darkness treatment benefited to callus growth and all-light was superior to all-darkness treatment. Inducing callus with culture media of glucose as carbohydrate source at 30℃、28℃、25℃ and 23℃ conditions, callus production significantly decreased with temperature lowdown; however inducing callus with culture media of sucrose as carbohydrate source at 30℃、28℃、25℃ and 23℃ conditions, callus production increased within temperature range of 25～28℃ and decreased at a range of over 30℃temperature or down to 23℃. Under light and lower temperature condition callus was easier to differentiate.