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中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (10): 63-63.

所属专题： 生物技术； 园艺

• 目次 • 上一篇    下一篇

辣椒细胞质雄性不育系离体培养植株再生研究

邓明华,,文锦芬,邹学校,杨 德,朱海山,周群初,刘志敏   

  • 出版日期:2005-10-05 发布日期:2005-10-05

In Vitro Plant Regeneration of Leaf Culture of Pepper CMS

Deng Minghua,, Wen Jinfen, Zou Xuexiao, Yang De, Zhu Haishan, Zhou Qunchu, Liu Zhimin   

  • Online:2005-10-05 Published:2005-10-05

摘要： 以辣椒细胞质雄性不育系9704A和8214A为试材，取无菌苗真叶进行培养。研究了不同浓度的IAA,6-BA和GA3对不定芽的诱导和伸长作用，结果表明：MS+6-BA5.0 mg/L +IAA1.0 mg/L有利于不定芽的诱导。MB+IAA1.0 mg/ L +6-BA3.0 mg/L + GA32.0 mg/L +AgNO310.0 mg/L有利于芽的伸长。将2.0 cm以上的芽转入生根培养基诱导不定根发现：1/2Ms+IAA0.3~0.5 mg/L有利于不定根的诱导。

Abstract: In this paper, using leaves from the seedling of pepper CMS 9704A and 8214A as experiment material ,effects of different content of IAA ,6-BA and GA3 on shoots regeneration and elongation were studied .Though selected : MS+6-BA5.0 mg/L +IAA1.0 mg/Lwas favoured for shoots regeneration. Shoots elongated well on MB+IAA1.0 mg/L+6-BA3.0 mg/L+GA32.0 mg/L +AgNO310.0 mg/L。1/2Ms+IAA0.3~0.5 mg/L were fit for root growth.