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中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (12): 292-292.

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中草药提取物对桃褐腐菌（Monilinia fructicola）抑制作用增效组合筛选

冯哓元,,孔 苗,李文生,姜微波   

  • 出版日期:2005-12-05 发布日期:2005-12-05

Screening of Combined Chinese Herb Extracts for the Fungitoxicity against Monilinia fructicola

Xiaoyuan Feng，；Miao Kong；Wensheng Li，Weibo Jiang   

  • Online:2005-12-05 Published:2005-12-05

摘要： 用菌丝生长速率法测定了虎杖、黄柏、厚朴、松萝等4种中草药提取物对桃褐腐菌的抑制作用，经线性回归计算50%抑制时的有效浓度（EC50）分别为133.8μg/ml、40.6μg/ml、90.6μg/ml、3.4μg/ml。根据EC50，用等效线法相加作用线的6等分点设置配比，共毒因子法判断增效标准。结果表明，各种配比的混配剂中黄柏+松萝为最佳增效作用组合，其中以8：1增效作用最显著。

Abstract: Inhibitory effect of Chinese herb extracts Polygonum cuspidatum，phellodendron Chinese schneid，Magnolia officinalis，Usnea longissima Ach. were determined by the growth rates of mycelium of Monilinia fructicola in vitro. By line regression，50% of Effective Concentration (EC50) of Chinese herb extracts were 133.8μg/ml, 40.6μg/ml, 90.6μg/ml and 3.4μg/ml respectively. According to EC50，the five ratios were determined by the evidence of six equal-part points of additive action line, and synergism was judged by the method of co-toxicity factor. The result showed that the plant extracts of phellodendron Chinese schneid and Usnea longissima Ach. with combined ratio of 8:1were the best mixtures for controlling M. fructicola.