多位点生物杀虫毒素BtA害虫敏感性的研究

摘要/Abstract

摘要： 多位点生物杀虫毒素BtA是通过氨基-羧基偶联剂EDC将两种生物毒素[Bacillus thuringiensis（Bt）晶体原毒素和阿维菌素]进行键合反应，形成的具有多个作用位点的生物藕合产物。通过研究小菜蛾（Plutella xylostella L.）对BtA敏感性的消减和恢复，以及不同地域小菜蛾对其敏感性的差异，来初步评估多位点生物杀虫毒素BtA害虫敏感性变化的规律。试验结果表明：小菜蛾室内相对敏感种群对BtA以及它的反应底物Bt伴胞晶体和阿维菌素的敏感性稳定（LC50为0.0004~0.0020mg/ml）。在三种生物杀虫剂的选择压力之下小菜蛾敏感性下降，2代后对BtA抗性比的增长（2.29）显著地低于Bt伴胞晶体(8.35)和阿维菌素(22.5)。小菜蛾田间种群在脱离杀虫剂的选择压力后敏感性会增加，2代后对BtA的敏感性显著地增加了4.7倍，高于对Bt伴胞晶体的1.28倍和对阿维菌素的2.00倍。福建省七个地市小菜蛾表现出敏感性的多态性，对BtA、Bt伴胞晶体和阿维菌素的抗性比分别为1.18~6.85、1.22~23.93和1.58~4.77。聚类分析结果在福建省以中部地区的小菜蛾具有相对较低水平的敏感性。说明多位点生物杀虫毒素BtA有助于延缓抗性的产生、降低抗性和迅速恢复害虫敏感性的作用。

Abstract: Multi-bioinsecticidal-toxin BtA is a bioconjugation product with multiple acting-sites, formed by biochemical bond-linking reaction between two biotoxins, protoxin of Bacillus thuringiensis and Abamectin, through the amino and carboxyl conjugator EDC. The variation of pest susceptibility to Multi-bioinsecticidal-toxin BtA was evaluated by studying the decline and recovery of susceptibility of Plutella xylostella L. to BtA and the susceptibility diversity of P. xylostella populations collected from different regions. The results showed that the susceptibilities of the relatively susceptible lab population were stable to BtA and its two reactants, Bt crystal and Abamectin, with LC50s of 0.0004-0.0020mg/ml. Under the selection pressure, the pest susceptibility reduced, with Resistance Ratio (RR) of 2.29 to BtA after 2 generations comparing with 8.35 and 22.5 to Bt crystal and Abamectin respectively. While the field population was released from the selection pressure, the pest susceptibility increased 4.7-fold to BtA after 2 generations in comparison to 1.28-fold Bt crystal and 2.00-fold to Abamectin. The P. xylostella from seven regions of Fujian province represented polymorphism of susceptibility, while the RRs to BtA, Bt crystal and Abamectin were 1.18~6.85, 1.22~23.93 and 1.58~4.77 respectively. The result of cluster analysis displayed that P. xylostella in middle part of Fujian province had lower susceptibility. It was concluded that the Multi-bioinsecticidal-toxin BtA could help to delay the resistance development, reduce the resistance and rapidly recovery of pest susceptibility.

朱育菁,,刘波,Cetin Sengonca,阮传清,林抗美,冒乃和. 多位点生物杀虫毒素BtA害虫敏感性的研究[J]. 中国农学通报, 2005, 21(12): 316-316.

Zhu Yujing,, Liu Bo, Cetin Sengonca, Ruan Chuanqing, Lin Kanmei, Mao Naihe,. Study on Pest Susceptibility to Multi-bioinsecticidal-toxin BtA[J]. Chinese Agricultural Science Bulletin, 2005, 21(12): 316-316.

参考文献

1 张国洲.害虫抗药性及其治理[J].安徽农业科学,2002,30(4):512~514
2 Shelton A M, Kroening M K, Eigenbrode S D, et al. Diamondback moth (Lepidoptera: Plutellidae) contamination of cabbage transplants and the potential for insecticide resistance problems[J]. J Entomol Sci, 1996, 31: 347~354
3 Iqbal, M., Wright, D. J. Evaluation of resistance, cross-resistance and synergism of abamectin and teflubenzuron in a multi-resistant field population of Plutella xylostella (Lepidoptera: Plutellidae) [J]. B Entomol Res, 1997, 87: 481~486
4 Zhao, J, Collins H L, Tang J D, et al. Development and characterization of diamondback moth resistance to transgenic broccoli expressing high levels of Cry1C[J]. Appl Environ Microb, 2000, 66: 3784~3789
5 刘波,朱育菁,Sengonca C,等.害虫抗药性治理策略——多位点杀虫毒素的研究[J].农药,2004,43(11): 492~496
6 Liu B, Sengonca C. Conjugation of δ-endotoxin from Bacillus thuringiensis with abamectin of Streptomyces avemitilis as a new type of biocide GCSC-BtA in control of agricultural insect pests[J]. J Pest Sci. 2003, 76: 44~49
7 Liu B, Sengonca C. Biotechnological development of GCSC-BtA as a new type of biocide[M]. Germany: Cuvillier Verlag G?ettingen, 2004.1~3
8 刘波,苑宝玲,Sengongca C,等.高效生物杀虫剂藕合技术的研究[J].中国生物防治,2000,12(增刊): 28~34
9 Finney D J. Probit Analysis[M]. 3rd edn. Cambridge Univ Press, Cambridge, UK, 1971.333
10 唐启义,冯明光.实用统计分析及其计算机处理平台[M].北京:中国农业出版社,1997.46~70
11 黄剑,吴文君.小菜蛾抗药性研究进展[J].贵州大学学报(自然科学版),2003,20(1): 97~104
12 Liu, Y, Tabashnik B E, Liu Y B. Inheritance of resistance to the Bacillus thuringiensis toxin Cry1C in the diamondback moth[J]. Appl Environ Microb, 1997, 63:2218~2223
13 何婕和,张雪燕.小菜蛾对阿维菌素的抗性形成规律[J].西南农业学报,2000,13(2): 67~70
14 李凤良,李忠英,韩招久,等.小菜蛾对溴氰菊酯的抗性消退与抗性恢复动态[J].植物保护学报,2004,31(1): 79~84
15 吴刚,尤民生,赵士熙.小菜蛾抗性稳定性及抗性治理对策研究[J].农药学学报,2001,3(1): 83~86