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中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (12): 330-330.

所属专题： 生物技术； 园艺

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Harpins蛋白防治黄瓜霜霉病机理研究 Ⅰ黄瓜接种Harpins蛋白后PAL酶活性的变化

申宏波,,文景芝,马成云,苗兴芬,马淑梅,郑天琪,丁俊杰,   

  • 出版日期:2005-12-05 发布日期:2005-12-05

The Research of the Mechanism that Using Harpins Protein to Prevent and Cure Cucumber Frost Mildewed Disease Ⅰ the Activity variety of PAL Enzyme after Inoculating Harpins Protein.

Shen Hongbo.,Wen Jingzhi,Ma Chengyun,Miao Xingfen,Ma Shumei, Zheng Tianqi, Ding Junjie   

  • Online:2005-12-05 Published:2005-12-05

摘要： 通过分析棚室黄瓜在喷施不同浓度的Harpins蛋白后，叶片中苯丙氨酸解氨酶(PAL)活性变化的规律，探讨了Harpins蛋白防治黄瓜霜霉病的机理，研究结果表明：黄瓜在喷施Harpins蛋白后不同时期PAL活性变化是不一样的，0.005g/L浓度的Harpins蛋白接种后，叶片中苯丙氨酸解氨酶(PAL)活性变化的规律与空白对照无显著差异，0.015、0.045g/L浓度的Harpins蛋白接种后，叶片中苯丙氨酸解氨酶(PAL)活性变化的规律与空白对照差异显著。黄瓜接种Harpins蛋白后PAL活性的变化，可作为一种生化指标来作为Harpins蛋白防治黄瓜霜霉病的一个证据。

Abstract: Through Analyzing the phenylalanine ammonialyase(PAL) activity variety regularity of the leaves after sprayed Harpins protein with different density on indoor cucumber, the thesis probed the mechanism of preventing and curing cucumber with Harpins protein,the result showed：the PAL activity variety during different periods after spraying Harpins protein is different, the PAL activity variety regularity in the leaves has no significant difference, comparing with the check experiment after inoculating Harpin protein with 0.005g/L; however, when inoculating Harpin protein with 0.015g/L,0.045g/L, that of difference is significant , comparing with the check experiment , The PAL activity variety after inoculating Harpins protein can be used as a physiological and chemical index sign, which can be used as a testimony of protecting and curing cucumber frost mildewed disease with Harpins protein.