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中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (12): 403-403.

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罗汉果遗传转化受体再生体系的建立及发根农杆菌转化初探

曾黎辉,吴金寿,柯石山,刘 芳   

  • 出版日期:2005-12-05 发布日期:2005-12-05

Establishment of the Explant Regeneration System for Genetic Transformation and Transformation by Agrobacterium rhizogene of Siraitia grosvenorii（Swingle）C. Jeffrey

Zeng Lihui, Wu Jinshou, Ke Shishang, Liu Fang   

  • Online:2005-12-05 Published:2005-12-05

摘要： 以罗汉果品种“青皮果”试管苗的茎段和叶片为外植体，研究适合于遗传转化的受体再生系统，并进行了发根农杆菌转化的初步研究。试验结果表明茎段的再生能力强于叶片，且对农杆菌敏感，是进行遗传转化合适的受体材料。在MS基本培养基附加BA 1mg/L、KT 1mg./L、NAA 0.2mg./L、蔗糖30 mg/L培养基中，20d龄的茎段不定芽诱导率为100%，平均每个外植体诱导的不定芽数达到9.7个，移苗成活率90%以上；外植体培养前的割伤处理有利于提高出芽数；发根农杆菌转化茎段诱导出毛状根。罗汉果毛状根的诱导和培养将为罗汉果药用成分的研究和开发提供新的途径。

Abstract: Stem segments and leafy discs from in vitro grown plantlets of Siraitia grosvenorii（Swingle）C. Jeffrey cv.Qingpinguo were used as explants,the regeneration system of recipient material for genetic transformation was developped. Transformation mediated by Agrobacterium rhizogene was also explored in this experiment. The results showed that the regeneration ability of stem was better than leafy and stem segments were sensitive to Agrobacterium . Stem segments were good recipent material for gene transformation. On the MS basic medium with 1 mg/ LBA, 1 mg/ LKT, 0.2 mg/ L NAA and 30 mg/L sugarose, the frequency of bud induction of 20-day-old stem segments was 100%, every stem segments produced 9.7 buds in average and the survival rate of transferred plantlets was over 90%. Injury to explants improved the number of induced bud. Hairy roots were formed from stem segments infected by Agrobacterium rhizogene. The induction and the culture of hairy root of Siraitia grosvenorii（Swingle）C. Jeffrey will be helpful for the study and development of its medicinal functions.