桔梗提取物的主要成分和抗氧化性的测定

doi:10.11924/j.issn.1000-6850.casb17070092

中国农学通报 ›› 2017, Vol. 33 ›› Issue (31): 139-144.doi: 10.11924/j.issn.1000-6850.casb17070092

桔梗提取物的主要成分和抗氧化性的测定

孙晓春

陕西中医药大学陕西省中药资源产业化协同创新中心

收稿日期:2017-07-19 修回日期:2017-08-05 接受日期:2017-08-30 出版日期:2017-11-27 发布日期:2017-11-27
通讯作者: 孙晓春
基金资助:
陕西省教育厅自然科学专项项目“桔梗不同部位的化学成分及关键基因的差异表达研究”(15JK1206)；陕西省教育厅项目“春笋计划” （[2016]16 号）。

Detection of Main Components and Antioxidant Activity of Platycodon grandiflorum Extracts

Received:2017-07-19 Revised:2017-08-05 Accepted:2017-08-30 Online:2017-11-27 Published:2017-11-27

摘要/Abstract

摘要： 为评价桔梗提取物中主要成分的含量和体外抗氧化性，通过回流提取法制备桔梗水提物和醇提物，水提醇沉法制备桔梗多糖；高效液相色谱法检测桔梗皂苷D含量，亚硝酸钠-硝酸铝比色法检测总黄酮含量，硫酸-苯酚法检测多糖含量；采用分光光度法检测不同提取物清除羟基和DPPH 自由基的能力。结果表明：安徽和吉林产地桔梗醇提物中总黄酮在生药中含量分别为1.35%和1.30%，均高于陕西桔梗1.08%，安徽和吉林产地桔梗醇提物对DPPH·和·OH的清除率均高于陕西桔梗醇提物；安徽、吉林和陕西桔梗多糖在生药中含量分别为0.45%、0.39%和0.35%。3 个产地桔梗多糖对DPPH·和·OH的清除作用安徽最高，吉林次之，陕西最低。桔梗醇提物对DPPH·和·OH的清除作用均高于桔梗水提物和桔梗多糖对DPPH·和·OH的清除作用。试验认为，桔梗醇提物对于DPPH·和·OH均具有较好的清除作用，以期为开发相关的抗氧化性产品提供参考。

Abstract: The paper aims to evaluate main components and antioxidant activity of Platycodon grandiflorum extracts. Water and ethanol extracts were prepared by reflux extraction, polysaccharide was prepared by water extracting- alcohol precipitating method. Platycodin D content was detected by HPLC method, spectrophotometry was used to detect the content of total flavonoids and polysaccharide. The antioxidant activities of the extracts were detected based on the clearance of hydroxyl radical and DPPH · model. The content of total flavonoids in ethanol extracts from Anhui, Jilin and Shaanxi was 1.35%, 1.30% and 1.08%, respectively. The clearance rate for scavenging DPPH· and ·OH of ethanol extracts from Anhui and Jilin was higher than that from Shaanxi. The content of polysaccharide in water extracts from Anhui, Jilin and Shaanxi was 0.45%, 0.39% and 0.35%, respectively. The clearance rate of polysaccharide for scavenging DPPH· and ·OH from Anhui was the highest, that from Jilin was the next, and that from Shaanxi was the lowest. The clearance rate of P. grandiflorum ethanol extracts for scavenging DPPH· and ·OH was higher than that of water extracts and polysaccharide. Ethanol extracts have good in vitro antioxidant properties and provide some reference for the development of related antioxidant products.

中图分类号:

R282.6

孙晓春. 桔梗提取物的主要成分和抗氧化性的测定[J]. 中国农学通报, 2017, 33(31): 139-144.

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桔梗提取物的主要成分和抗氧化性的测定

Detection of Main Components and Antioxidant Activity of Platycodon grandiflorum Extracts

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