MSTN及FGF5基因编辑不改变绒山羊母羊同期发情能力

doi:10.11924/j.issn.1000-6850.casb17080082

中国农学通报 ›› 2018, Vol. 34 ›› Issue (9): 124-126.doi: 10.11924/j.issn.1000-6850.casb17080082

所属专题：生物技术；畜牧兽医

MSTN及FGF5基因编辑不改变绒山羊母羊同期发情能力

朱海鲸,刘锦旺,李陇平,黄帅,屈雷

榆林学院陕西省陕北绒山羊工程技术研究中心,榆林学院陕西省陕北绒山羊工程技术研究中心,榆林学院陕西省陕北绒山羊工程技术研究中心,榆林学院陕西省陕北绒山羊工程技术研究中心,榆林学院陕西省陕北绒山羊工程技术研究中心

收稿日期:2017-08-18 修回日期:2017-11-28 接受日期:2017-12-20 出版日期:2018-03-21 发布日期:2018-03-21
通讯作者: 朱海鲸
基金资助:
陕西省农业科技创新与攻关项目“陕北白绒山羊性别控制技术研究”(2016NY-105)；陕西省重大科技统筹项目“榆林羊产业提质增效关键技术研究与产业化开发示范”(2014KTDZ02-01)；榆林学院高层次引进人才科研启动项目“陕北白绒山羊雄性生殖干细胞发育分化规律的研究” (16GK06)。

MSTN and FGF5 Gene Editing Does Not Affect Estrus Synchronization of Cashmere Goats

Received:2017-08-18 Revised:2017-11-28 Accepted:2017-12-20 Online:2018-03-21 Published:2018-03-21

摘要/Abstract

摘要： 基因编辑动物是研究动物生产性状及基因功能的良好载体,目前关于各类基因编辑动物的研究广泛开展。然而,基因编辑动物繁殖性能方面是否与野生型个体具有明显差异则尚未见报道。本研究拟通过比较野生型绒山羊与MSTN、FGF5基因编辑绒山羊对同期发情处理的响应差异,确定其与野生型绒山羊同期发情效率的差异。以野生型和MSTN、FGF5基因编辑陕北白绒山羊为研究对象,在繁殖季节采用同种处理方式(CIDR + PG)对试验羊只进行处理,研究两类羊只在繁殖季节同期发情效率的差异。结果表明,野生型绒处理后同期发情率为100%,基因编辑绒山羊处理后同期发情率也为100%,两组无差异；比较MSTN和FGF5基因编辑的羊只在处理后同期发情效率的差异,发现两组无差异,与野生型绒山羊相比同样无差异。本研究结果证实了MSTN及FGF5基因编辑后不影响母羊的繁殖性能,为基因编辑动物的推广应用提供了试验基础。

Abstract: In this study, we aim to compare the responses of wild-type cashmere goats withMSTN- orFGF5-editing cashmere goats on the hormones for estrus synchronization, then find out the difference of estrus synchronization efficiency between the wild-type and the gene-editing cashmere goats. The goats were treated with the same method (CIDR + PG) in the breeding season. The results showed that the estrus synchronization rate of the wild-type cashmere goats was 100 % after the treatment, and that of the gene-editing goats was also 100% . Moreover, the MSTN- and FGF5- editing goats showed no significant difference in the estrus synchronization efficiency, and also showed no significant difference in that with the wild-type goats. The results confirmed thatMSTN andFGF5 gene editing did not affect the reproductive performance of cashmere goats. The results provide an experimental basis for the application of gene editing animals.

朱海鲸,刘锦旺,李陇平,黄帅,屈雷. MSTN及FGF5基因编辑不改变绒山羊母羊同期发情能力[J]. 中国农学通报, 2018, 34(9): 124-126.

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MSTN及FGF5基因编辑不改变绒山羊母羊同期发情能力

MSTN and FGF5 Gene Editing Does Not Affect Estrus Synchronization of Cashmere Goats

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