Abstract: The study aims at exploring the role of the tail lipids of Altay sheep in wound healing of skin, and comparing its healing effect with that of the cattle lipids and commercial products. Forty Kunming mice weighing 31 ± 1 g, half male and half female were selected. The wound models of mice skin were constructed, and then these mice were divided into four groups (sheep lipids group, cattle lipids group, commercial product group and blank control group). The wounds healed well after different treatments, the wound healing ability of female mice were better than that of the males, and the wound healing rates of female mice were: sheep lipids > cattle lipids > commercial product > blank control group, whereas those of the male mice were: sheep lipids > commercial product > cattle lipids > blank control group. The results of skin histopathology showed that compared with the blank control group, in the other three groups, the quantities and distribution of adipose cell, collagen fibrils and other cells in wound repair tissue were different. Both the sheep tail lipids and the cattle lipids have effects on repairing skin injury, and the sheep tail lipids have the better one. The sheep tail lipids can accelerate the skin repair by promoting collagen fibers producing.