p38MAPK信号通路选择性调节FSH对甾体生成
的诱导作用研究

中国农学通报 ›› 2006, Vol. 22 ›› Issue (2): 9-9.

p38MAPK信号通路选择性调节FSH对甾体生成的诱导作用研究

赵云焕,张自富,赵聘

出版日期:2006-02-05 发布日期:2006-02-05

Study on FSH-activated p38MAPK Pathway Differently Regulated the Progesterone and Estrogen Synthesis in the Cultured Primary GCs

Zhao Pin, Zhang Zifu, Zhao Yunhuan

Online:2006-02-05 Published:2006-02-05

摘要/Abstract

摘要： 利用乙烯雌酚（DES）处理23日龄SD大鼠，分离卵巢颗粒细胞(GC)进行无血清培养。结果表明，FSH (50ng/m1) 处理GC，可迅速激活有丝分裂原蛋白激酶（p38 MAPK），FSH处理5min便可观察到磷酸化的p38 MAPK；FSH处理30min，磷酸化的p38 MAPK水平达到最高;向培养液中加入H89(蛋白激酶A抑制剂，10μM)，则显著抑制了FSH对p38 MAPK的激活作用，提示这种激活作用依赖于蛋白激酶A(PKA)。用 SB203580(p38MAPK抑制剂，20μM)抑制p38 MAPK激活，则进一步提高了FSH对孕酮和甾体生成快速调节蛋白（StAR）的诱导作用，同时降低了FSH对雌激素生成的促进作用(p<0.01)。RT-PCR结果显示：抑制p38 MAPK活性后，FSH对StAR mRNA刺激作用明显增强，但对细胞色素P450芳香化酶（P450arom）mRNA的诱导作用却减弱了(p<0.05)。激光共聚焦和蛋白印迹结果显示：在GC中，StAR蛋白主要分布在线粒体中；与对照组相比，FSH显著提高了StAR的荧光强度和蛋白水平；抑制p38MAPK活性则增强了FSH对StAR蛋白表达的诱导作用。

Abstract: GCs were obtained from the ovaries of activity of DES-treated immature rats and cultured in serum-free medium. FSH receptor occupancy lesded to phosphorylation/activation of p38 MAPK in time-dependent manner. Phosphorylated p38 MAPK by FSH was observed within 5 min and reached the highest level at 30 min. Moreover, such activation was protein kinase A-dependent as indicated by the results using specific inhibitors.Interestingly.inhibition of p38 MAPK acitivity with SB203580 augmented estradiol production at the same time (p<0.01). RT-PCR data showed that inclusion of SB203580 in the FSH-stimulated FSH-induced STAR mRNA Production, while decreased the FSH-stimulated P450arom mRNA expression of STAR in mitochondria than FSH treatment alone.

赵云焕,张自富,赵聘. p38MAPK信号通路选择性调节FSH对甾体生成的诱导作用研究[J]. 中国农学通报, 2006, 22(2): 9-9.

Zhao Pin, Zhang Zifu, Zhao Yunhuan. Study on FSH-activated p38MAPK Pathway Differently Regulated the Progesterone and Estrogen Synthesis in the Cultured Primary GCs[J]. Chinese Agricultural Science Bulletin, 2006, 22(2): 9-9.