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中国农学通报

中国农学通报 ›› 2006, Vol. 22 ›› Issue (2): 292-292.

所属专题： 园艺

• 目次 • 上一篇    下一篇

小美石斛茎段培养的研究

林泋君   

  • 出版日期:2006-02-05 发布日期:2006-02-05

Stem-segment Culture and Tube Propagation of Dendrobium bellatulum Rolfe

Lin Huijun   

  • Online:2006-02-05 Published:2006-02-05

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摘要： 用小美石斛茎段作外殖体，进行试管繁殖。筛选出各培养阶段适宜的培养基，(1)腋芽萌生MS + 6-BA 5mg/L+NAA 0.4 mg/L；(2)继代增殖 MS + 6-BA 2mg/L+NAA0.2mg/L，三种培养基MS，1/2MS，N6，以MS增殖效果最好，(3)适宜的生根培养基为1/2MS + NAA0.1mg/L+PP333 0.1mg/L 其中平放的芽块生根速度，生根质量和生根率均高于直立放置的。

Abstract: The stem-segment with axillary buds of Dendrobium bellatulum Rolfe as the explants, were cultured in tubes. The results of experiments suggested that appropriate media for respective culture stages were: (1) The germinaton of axillary buds: Ms + 6-BA5mg/L +NAA0.4mg/L (2) The differentiation and subculture Ms + 6-BA2 mg/L +NAA0.2 mg/L (3)Rootage: 1/2 MS+NAA0.2mg/L+PP333 was optional basal medium for proliferation , the rooting speed, number and percentage of adventitionous buds placed the medium levelly were significantly higher than thouse placed uprightly.