In this study, genetic background of Jialing16 and their parents are analyzed by the AFLP (Amplified Fragment Length Polymorphism), from which the genetic distance, similarity coefficient and UPGMA dendrogram of the materials are obtained. These will provide reliable evidence for the selection and tissue mating of parents. it is found the similarity coefficient between Xiqing1（4x；♀）and Yu2（2X；♂）, which are parents of Jialing16,is 0.6607. These will be helpful in the breeding of new artificial triploid mulberry with strong heterosis and multiploid superiority.
