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中国农学通报 ›› 2006, Vol. 22 ›› Issue (8): 74-74.

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一株耐碱根瘤菌的筛选与鉴定

汤 晖,隋新华,陈文新   

  • 出版日期:2006-08-05 发布日期:2006-08-05

Screening and Identification of Alkali-Tolerance Strains

Tang Hui, Sui Xinhua, Chen Wenxin   

  • Online:2006-08-05 Published:2006-08-05

摘要: 为探讨耐碱微生物的耐碱机制,自CCBAU根瘤菌菌库中筛选得到一株耐受pH11.5的根瘤菌,编号为CCBAU 81024。通过对其生理生化特征的测定、16SrDNA序列分析、DNA G+Cmol%测定以及DNA-DNA杂交,鉴定其为Sinorhizobium meliloti。为进一步的研究根瘤菌的耐碱机制提供了实验材料。

关键词: 大穗, 大穗, 一次枝梗, 千粒重, 粒/叶

Abstract: : A alkali-tolerance strain CCBAU 81024 was selected from CCBAU, which grows on TY medium at pH 11.5. Further analysis of primary physiological and biochemical characteristics, 16SrDNA nucleotide sequences blast, DNA G+Cmol% and DNA-DNA hybridization provided supporting evidence that the strains belongs to the Sinorhizobium meliloti and offered potential material for studies of the mechanism of alkali-tolerance in the rhizobia.

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