紫芦笋茎尖组培快繁技术研究

中国农学通报 ›› 2004, Vol. 20 ›› Issue (3): 190-190.

紫芦笋茎尖组培快繁技术研究

张元国刁家连李芳李保华李志萍

出版日期:2004-06-05 发布日期:2004-06-05

Study on Techniques for Shoot Tip Culture of Purple Asparagus

Zhangyuanguo,diaojialian,lifang,libaohua,lizhiping.

Online:2004-06-05 Published:2004-06-05

摘要/Abstract

摘要： 紫芦笋以茎尖为外植体，进行组织培养，其研究结果为：（1）启动培养基：MS+6-BA1.0mg/l；（2）增殖培养基：适合紫芦笋雄株增殖的培养基是：MS+NAA0.5mg/l +6-BA0.5mg/l,每切片平均发芽数可达2.5个;适合紫芦笋雌株增殖的培养基是：MS+6-BA0.3mg/l+NAA0.05mg/l,平均发生芽数可达2.1个；（3）生根培养基：改良MS+IBA1.0mg/l+KT0.2mg/l+NAA0.05mg/l，生根率高达85%；（4）过度移栽技术：选用3条根以上的组培苗在土5份十垤石3份的基质上移栽。

Abstract: The tissue culture of stem tip from purple Asparagus was studied.The results showed:(1)The suitable medium for induction culture was MS+6-BA1.0mg.L-1;(2)The suitable mutiplication medium for male plant of purple Asparaguswas MS+NAA0.5 mg.L-1+6-BA0.5 mg.L-1with multiplication coefficient 2.5; The suitable mutiplication medium for famale plant of purple Asparaguswas MS+NAA0.05 mg.L-1+6-BA0.3 mg.L-1with multiplication coefficient 2.1;(3)Rooting medium:Improved MS+NAA0.05 mg.L-1+IBA1.0 mg.L-1+KT0.2 mg.L-1 with rooting rate 85%;(4)Transition cultivating technique:Tissue culture seedseedings with more than 3 roots cultivated in the mixed soil according to soil/vermiculite rate 5/3.

张元国刁家连李芳李保华李志萍. 紫芦笋茎尖组培快繁技术研究[J]. 中国农学通报, 2004, 20(3): 190-190.

Zhangyuanguo,diaojialian,lifang,libaohua,lizhiping.. Study on Techniques for Shoot Tip Culture of Purple Asparagus[J]. Chinese Agricultural Science Bulletin, 2004, 20(3): 190-190.