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中国农学通报

中国农学通报 ›› 2006, Vol. 22 ›› Issue (12): 21-21.

所属专题： 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

高产复合酶益生菌的氮离子注入诱变选育

陈丽娟,张云华,王宝玉,孟秀丽,程茂基   

  • 出版日期:2006-12-05 发布日期:2006-12-05

Breeding of High-yield Compound Enzyme Probiotic Aspergillus niger by N+ Implantation

Chen Lijuan, Zhang Yunhua, Wang Baoyu, Meng Xiuli, Cheng Maoji   

  • Online:2006-12-05 Published:2006-12-05

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摘要： 以产复合酶益生菌黑曲霉ANO1为出发菌株，经多次N+注入诱变处理，结合平板透明圈法定向选育，得到高产变异菌株ANO2。结果显示，出发菌株ANO1酸性蛋白酶、纤维素酶和果胶酶的酶活显著提高，分别由71.6 IU/g、141.7 IU/g和264.8 IU/g提高到996.5 IU/g、940.4 IU/g和906.5 IU/g。变异菌株ANO2经传5代培养，产酶特性稳定。同时对原菌株和变异菌株的生物学特性进行了相关的研究，结果表明变异菌株的生物量和产酶量成正相关。

关键词: 肉仔鸡, 肉仔鸡, 投资规模, 数学模型

Abstract: A mutated strain ANO2 with high yield of compound enzymes was obtained from the strain ANO1 of Aspergillus niger by N+ implantation, and screened by using transparent zones of agar plate screening techniques. The results showed that activities of acid-protease, Cellulase and Pectinase in Aspergillus niger were raised signifcantly from 71.6 IU/g, 141.7 IU/g and 264.8 IU/g to 996.5 IU/g, 940.4 IU/g and 906.5 IU/g respectively. Characteristics of enzymes production in mutated strain Aspergillus niger AN02 were stabile by 5 times subculture. Biological characteristics of the original strain ANO1 and the mutant strain ANO2 were researched. Biomass and enzyme activity of the mutant strain ANO2 had positive correlation.