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中国农学通报

中国农学通报 ›› 2007, Vol. 23 ›› Issue (6): 72-72.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

猪Musclin基因片段克隆与序列分析

王伟杰,郭豫杰,李卫华,林茂旺,赵蕾,杨国宇   

  • 出版日期:2007-06-05 发布日期:2007-06-05

Molecular cloning and sequence analysis of porcine musclin

Wang Weijie, Guo Yujie, Li Weihua , Lin Maowang, Zhao Lei ,Yang Guoyu   

  • Online:2007-06-05 Published:2007-06-05

摘要： 【研究目的】克隆并分析猪musclin基因；【方法】肌肉组织提取总RNA，利用设计的引物进行RT-PCR，PCR产物与pMD19-T连接后转化E. coli DH5α，检测阳性克隆并测序；【结果】克隆的猪musclin基因片段与人、大鼠、小鼠同源性分别为86%、78%、75%，预测的氨基酸序列含有“KKKR”结构和与小鼠ANP、BNP、CNP蛋白的同源性区域；【结论】克隆了猪musclin基因片段并注册GenBank (Accession.EF369511)。

关键词: 反义RNA, 反义RNA, 猪传染性胃肠炎病毒, 逆转录病毒载体, 细胞病变

Abstract: 【Objective】To clone and analysis the porcine musclin.【Method】Total RNA was extracted from muscle tissue and mRNA sequence of gene were amplified by RT-PCR using two designed primers. The PCR products were ligated into the pMD-19Tvector, and then transformed into competent cells of E. coli DH5α.The sequence was analyzed to identify the recombinant plasmid.【Results】Identity analysis showed that the musclin nucleotide sequence shared 86%, 78% and 75% homology with that of human , rat and mouse. The predicted peptide contained a “KKKR” motif and the region homologous to mouse ANP, BNP, and CNP.【Conclusion】The porcine partial musclin gene was successfully cloned in present study and the sequence has been submitted to GenBank(Accession.EF369511).

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