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中国农学通报 ›› 2007, Vol. 23 ›› Issue (9): 12-12.

所属专题: 生物技术 园艺

• 农学 农业基础科学 • 上一篇    下一篇

柠檬酸和苹果酸对小白菜种子萌发和细胞膜透性的影响

  

  • 出版日期:2007-09-05 发布日期:2007-09-05

Effects of Citrate and Malate on the Seed Germination and Memberane Permeability of Pakchoi

Xing Suzhi, Wang Jianfei, Cai Dan   

  • Online:2007-09-05 Published:2007-09-05

摘要: 【研究目的】研究不同浓度的柠檬酸和苹果酸溶液浸种对小白菜种子萌发、幼芽生长及种胚细胞膜透性的影响。【研究方法】将有机酸浸泡后的小白菜种子,摆放在培养皿中恒温培养,测定发芽率等指标;用电导率仪测定小白菜种子细胞膜透性。【研究结果】(1)柠檬酸浓度达64mmol/L,苹果酸浓度达32mmol/L时,有机酸处理的种子发芽率和发芽指数显著低于对照;(2)有机酸浓度为128 mmol/L时,柠檬酸处理的小白菜胚根重、胚根长和茎长分别是对照的50%、60.5%和47.4%;苹果酸处理对应的值分别为26.8%、44.1%和30.0%;(3)有机酸对小白菜种子细胞膜透性影响显著,且随处理浓度增加细胞膜透性逐渐增加。【研究结论】两种有机酸对小白菜种子萌发和幼芽的生长均有抑制作用,且浓度相同时,苹果酸的抑制作用比柠檬酸强

Abstract: 【OBJECTIVE】The effects of citrate and malate on the pakchoi seed germination, young buds growth and embryo cell membrane permeability. 【METHOD】The pakchoi seeds soaked with different concentration solution of citrate and malate were placed in culture dishes, then were to measure germination rate and other indexes. Embryo cell membrane permeability was determined using inductivity measurer.【RESULTS】(1) when citrate concentration was up to 64 mmol/L, or malate concentration to 32 mmol/L, the germination rate and index of pakchoi seed soaked in organic acids solution were notably lower than contrast. (2) When organic acids concentration were 128 mmol/L, the weight and length, stem length of pakchoi soaked citrate solution was 50%, 60.5% and 47.4% of contrast respectively. Correspondingly, the values soaked malate solution was 26.8%, 44.1%, 30.0% respectively. (3) The effects of organic acids on pakchoi seed memberane permeability were significant, and were stronger with increased of organic acids concentration. 【CONCLUSION】The two organic acids all inhibited the seed germination and young buds growth of pakchoi. Moreover, the inhibition of malate was stronger than of citrate at the same concentration.

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