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中国农学通报

中国农学通报 ›› 2007, Vol. 23 ›› Issue (11): 228-228.

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土壤样品中有效氮的化学发光法测定

刘炎超,孟 磊,高向阳,贾 玮,王志荣,孙伟涛   

  • 出版日期:2007-11-05 发布日期:2007-11-05

A Chemiluminescence Method for Determination of Available Nitrogen in Soil Samples

Liu Yanchao, Meng Lei, Gao Xiangyang, Jia Wei, Wang Zhirong, Sun Weitao   

  • Online:2007-11-05 Published:2007-11-05

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摘要： 建立了一种快速简便的测定土壤中铵态氮的方法。土壤中的有效氮加碱蒸馏，用硫酸吸收，全部转化为铵态氮，NH4+在碱性溶液中能还原ClO－，该反应和化学发光反应ClO－－Luminol相耦合，通过测定剩余的ClO－来达到间接测定NH4+的目的。该方法的检测下限达到10-3mg/kg，线性范围0.01~1.5mg/kg，相关系数为-0.9995，加标回收率在85.8%~94.5%之间，RSD＜2.7%(n=5)，完全能满足测定土壤中有效氮的需要。

关键词: 除草剂安全剂, 除草剂安全剂, 绿磺隆, 解毒效果。

Abstract: A luminol-ClO－ chemiluminescence（CL）method for the determination of available nitrogen in soil with high sensitivity was developed. It was found that NH4+ could reduce ClO－ in the base solution. A new sensitive and selective CL method for the determination NH4+ in the dry soil was found based on the reductive reaction. The linear-response range of the method was 0.01~1.50mg/kg with a linear correlation coefficient of–0.9989.The detection limit was 1×10-3mg/kg. The RSD was less than 2.7%(n=5). The recovery of the method was 85.8%~94.5%. The method was applied to the detection of available nitrogen in soil samples with satisfactory results.

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