ABT和CaCN2对葡萄插条萌芽生根及一些生理生化指标的影响

摘要/Abstract

摘要： 以无核白葡萄一年生枝条为试材，研究ABT和CaCN2对葡萄插条萌芽生根及生理生化代谢的影响。结果表明，ABT处理的葡萄硬枝插条生根早，生根率高，生根数多，不定根生长健壮；CaCN2处理的葡萄插穗萌芽早，萌芽率高，萌芽后枝条平均生长量大；ABT＋CaCN2处理的萌芽率和生根率介于二者之间，但均与对照差异显著(P≤0.05)，与ABT、CaCN2单独处理差异不显著。ABT和ABT＋CaCN2处理能促使插穗中淀粉向可溶性糖的转化，从而促进插穗生根。IAAO、PPO与葡萄扦插生根过程有密切关系，其变化因生根时期不同而不同。总的趋势是前期高活性的IAAO有利于降解体内高浓度的IAA，促进根的诱导；后期低活性的IAAO有利于保护IAA免受分解，促进根的生长。PPO在不定根形成时，活性迅速上升，从而促进生根。

关键词: 南欧蒜, 南欧蒜, 普通大蒜, 营养组分

Abstract: The rooting and physiological biochemistry metabolize changes were studied during rooting of the hardwood of Thompson seedless grape (Vitis vinifera L.) by treating of ABT and CaCN2 with water control. The results indicated that the cuttings by treating of ABT rooted earlier, rooting date and the number of roots more than other treatments, and the roots grew healthily. The cuttings by treating of CaCN2 buded earlier, and buding date and the length of branchs were more than others.Root rates and bud rates by treating of CaCN2 firstly , then by treating of ABT were between ABT and CaCN2, respectively, but there were significant difference with water control, and no significance ABT and CaCN2, respectively. Starch could change to soluble sugar easily by treating of ABT singly or both ABT and CaCN2, so promoting rooting. The activity of indoleacetic acid oxidase ( IAAO ) , polyphenol oxidase (PPO ) , as w ell as, the relationship between enzyme activities and rooting capacities of Thompson seedless grape cuttings was studied in this paper. There were the more relationship between rooting capacities and enzyme activities, which changed with different rooting time. During callus formatting, higher IAAO activities could release IAA in grape, benefiting inducing roots, but during root elongating, lower IAAO activities benefited to protect IAA been released, promoting roots growing. During the course of adventitious root formation, PPO activities increase quickly, promoting rooting.

中图分类号:

S663.1

文颖强,冯嘉玥,万春雁. ABT和CaCN2对葡萄插条萌芽生根及一些生理生化指标的影响[J]. 中国农学通报, 2007, 23(12): 270-270.

Wen Yingqiang, Feng Jiayue, Wan Chunyan. Effects of ABT and CaCN2 on Budding, Rooting and Some Physiological and Biochemical Metabolize of Grape(Vitis vinifera L. Cv. Thompson seedless) Cuttings[J]. Chinese Agricultural Science Bulletin, 2007, 23(12): 270-270.

参考文献

[1] 杨德龙,于作文,梁庆丰,等.内39号葡萄株系的离体快繁技术研究[J].果树学报, 2002,19(6):427-429.
[2] 贺普超.葡萄学[M].北京:中国农业出版社, 1999:37- 38.
[3] 田莉莉,方金豹,顾红,等.化学物质打破葡萄休眠的应用效果初报[J].西北植物学报, 2003,23(6):997-1000.
[4] 白先进,朱建华,彭宏祥,等.外源物质促进桂南巨峰葡萄结果母枝萌芽试验[J].中外葡萄与葡萄酒, 2004, (5):18-19.
[5] 于大永,王跃进,张剑侠,等.中国野生葡萄打破休眠研究[J].中外葡萄与葡萄酒,2003, (6):17-19.
[6] 刘会宁.不同浓度ABT1号生根粉对藤稔葡萄的扦插效应[J].特产研究,2003, 1: 45-47.
[7] 高俊凤.植物生理学实验技术[M].西安:西安图书出版公司,2000: 145-148.
[8] 陈建勋,王晓峰.植物生理学实验指导[M].广州:华南理工大学出版社, 1999: 56-57.
[9] 吉林农业大学,植物生理学实验指导[M].长春:吉林科技出版社, 2000: 205-206.
[10] Pacheco P, et al. Flavonoids as regulators and markers of root formation by shoots of Eucalyptus globulus raises in vitro[J].Plant Perox N ew slett,1995,(5):9-12.
[11] 宋丽红,曹帮华.光叶楮扦插生根的吲哚乙酸氧化酶、多酚氧化酶、过氧化物酶活性变化研究[J].武汉植物学研究,2005,23 (4) : 347- 350.
[12] Pires E J P, TerraM M , Pommer C V. Adjustment of ideal H2CN2 concentration for breaking dormancy of grapevine in less warm region [J].Acta Hortic,1995,395:169- 176.
[13] Uzun H I, Kuden A B, Dennis F G J. Effects of hydrogen cyanamide application at various time, during dormancy on phonological stages and fruit characteristics of grapes[J].Acta Hortic,1997,441:201-206.
[14] 陈嫣嫣,彭峰,夏冰. IBA和ABT对曼地亚红豆杉插穗生根的影响[J].植物资源与环境学报,2005,14(3):61-62.
[15] 李明,黄卓烈,谭绍满,等.吲哚乙酸处理桉树插条后氧化酶活性及同工酶变化与生根关系的比较研究[J].林业科学研究,2001,14(2):131- 140.