cDNA-AFLP技术及在差异表达基因克隆上的应用

摘要/Abstract

摘要： cDNA-AFLP技术是在AFLP技术基础上发展起来的主要用于研究基因差异的技术，具有高效可靠的优点，受到研究者的青睐，被广泛应用于包括植物在内的各类生物的基因表达的研究中。简要阐述了cDNA-AFLP技术的原理，重点讨论了操作过程中可能出现的问题和技术关键，并就该技术与其他差异表达技术进行了比较，以说明其在克隆差异表达基因上的优势，还简要列举了一些在抗病育种等领域的应用实例。

关键词: 屋顶花园, 屋顶花园, 屋顶绿化, 建筑微气候

Abstract: cDNA-AFLP technology, which is used mainly for studying genetic difference, and developed on AFLP technological foundation, and had the reliable advantage and high-efficient, is favored by researcher and widely used in the research of all kinds of biological gene expression including plant. This article elaborated briefly the cDNA-AFLP technology principle, discussed emphatically the issue and the technical key which in the operating process possibly appears, and have carried on the comparison on this technology with other difference expression technology, explained it expresses in the gene in the clone difference the superiority, has enumerated briefly some examples in disease resistant breeding application.

中图分类号:

Q781

于虹,丁国华,. cDNA-AFLP技术及在差异表达基因克隆上的应用[J]. 中国农学通报, 2007, 23(12): 77-77.

Yu Hong, Ding Guohua,. cDNA-AFLP Technique and Its Application in Cloning of Differentially Expressed Genes[J]. Chinese Agricultural Science Bulletin, 2007, 23(12): 77-77.

参考文献

[1] 曹仪植.植物分子生物学.北京:高等教育出版社,2002.184.
[2] Bachem C W B, Hoeven R S, Bruijin S M, et al. Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: analysis of gene expression during potato tuber development. Plant Journal, 1996, 9: 745-753.
[3] Bachem C W B, Oomen R J F J, Visser R G F, et al. Transcript imaging with cDNA-AFLP: a step-by-step protocol. Plant Molecular Biology Reporter, 1998, 16: 157-173.
[4] Liang P, Pardee A B. Differential display of eukaryotic messenger RNA by means of the ploymerase China reaction. Science, 1992, 257: 967-971.
[5] Diatchenko L, Chris Lau Y F, Campbell A P, et al. Suppression subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proceedings of the National Academy of Science of the United States of America, 1996, 93: 6025-6030.
[6] Breyne P, Dreesen R, Cannoot B, et al. Quantitative cDNA-AFLP analysis for genome-wide expression studies. Mol Gen Genomics, 2003, 269: 173-179.
[7] Aubry C, Ricoult C, Peltier D, et al, A gene encoding a germin-like protein, identified by a cDNA-AFLP approach, is specifically expressed during germination of Phaseolus vulgaris. Planta, 2003, 217: 466-475.
[8] Bove J, Lucas P, Godin B, et al. Gene expression analysis by cDNA-AFLP highlights a set of new signaling networks and translational control during seed dormancy breaking in Nicotiana plumbaginifolia. Plant Molecular Biology, 52005, 7: 593-612.
[9] Huang J, Bachem C, Jacobsen E, et al. Molecular analysis of differentially expressed genes during postharvest deterioration in cassava tuberous roots. Euphytica, 2001, 120: 85-93.
[10] Jeney A, Beki E, Mule G, et al. Identification of growth stage specific transcript profiles in Fusarium proliferatum by cDNA-AFLP analysis. European Journal of Plant Pathology, 2004, 110: 619-625.
[11] Li C W, Bai Y L, Jacobsen E, et al. Tomato defense to the powdery mildew fungus: differences in expression of genes in susceptible, monogenic-and polygenic resistance responses are mainly in timing. Plant Mol Biol, 2006, 62:127-140.
[12] Durrant W E, Rowland O, Piedras P, et al. cDNA-AFLP reveals a striking overlap in race-specific resistance and wound response gene expression profiles. The Plant Cell, 2000, 12(6): 936-977.
[13] Eckey C, Korell M, Leib K, et al. Identification of powdery mildew-induced barley genes by cDNA-AFLP: functional assessment of an early expressed MAP kinase . Plant Molecular Biology, 2004,55:1-15.
[14] Zheng X, Chen X, Zhang X, et al. Isolation and identification of a gene in response to rice blast disease in rice. Plant Mol Biol, 2004, 54(1):99-109.
[15] Bruggmann R, Abderhalden O, Reymond P, et al. Analysis of epidermis-and mesophyll-specific transcript accumulation in powdery milder-inoculated wheat leaves. Plant Mol Biol, 2005, 58(2): 247-267.
[16] Mao C Z, Yi M K, Yang L, et al. Identification of aluminium-regulated genes by cDNA-AFLP in rice: aluminium-regulated genes for the metabolism of cell wall components. Journal of Experimental Botany, 2004, 55(394):137-143.
[17] Cracium A R, Courbot M, Bourgis F, et al. Comparative cDNA-AFLP analysis of Cd-tolerant and –sensitive genotypes derived from crosses between the Cd hyperaccumulator Arabidopsis halleri and Arabidopsis lyrata ssp. Petraea. Journal of Experimental Botany, 2006, 57(12):2967-2983.