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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (8): 23-27.

所属专题： 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

β-激动剂多组分残留的酶免疫分析方法

杨正涛,张乃生,史利军,李 颖   

  • 收稿日期:2008-06-18 修回日期:2008-07-01 出版日期:2008-08-08 发布日期:2008-08-08

Yang Zhengtao, Zhang Naisheng, Shi Lijun, Li Ying   

  • Received:2008-06-18 Revised:2008-07-01 Online:2008-08-08 Published:2008-08-08

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摘要： 制备并筛选能和多种β-激动剂反应的簇特异性抗体，建立能同时检测多种药物的酶免疫分析方法，为研制多组分残留检测试剂盒奠定基础。选取沙丁胺醇、克伦特罗和多巴胺3种代表性β-受体激动剂，分别与钥孔血蓝蛋白（KLH）偶联作为免疫抗原，与牛血清白蛋白（BSA）偶联作为检测抗原，免疫家兔获取抗血清，ELISA检测抗血清交叉反应率，筛选交叉反应性最大的抗体作为簇特异性抗体，用于建立ELISA方法并制作标准曲线。经测定获得的3种抗血清，其中的抗沙丁胺醇抗血清除对沙丁胺醇具有100%的反应之外，还对克伦特罗具有128%的交叉反应性，具有部分簇特异性。用沙丁胺醇抗血清建立了同时适用于沙丁胺醇和克伦特罗的ELISA分析方法，为多组分残留检测试剂盒的研制奠定了基础。

Abstract: To produce group-specific antibodies againstβ-agonists and to establish enzyme-immunoassay for the production of multi-residue detection kit. Clenbuterol, salbutamol and ractopamine were conjugated to KLH and BSA respectively. Rabbit were injected to prepare antiserums. ELISA was established with the group-specific antibody that selected by test the across-reaction of these antiserums. ELISA for salbutamol and clenbuterol was established with the anti-salbutamol antibody which shows 100% and 120% across-reaction with salbutamol and clenbuterol. This immunoassay provided an analytical method and laid a foundation for multiresidue ELISA kit.

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